Larry A. Sklar

TL;DR: The preparation of polyene fatty acid membrane probes cis- and trans-parinaric acid and parinaroylphosphatidylcholines and their use in studies of several one- and two- component lipid systems are described and several rates are observed in the binding process which are interpreted as rapid outer monolayer uptake and a much slower process of interlamellar exchange.

TL;DR: It is suggested that the first two fatty acids bind side-by-side in an antiparallel fashion in domain III of human serum albumin in support of proposed structural models for albumin.

TL;DR: The use of fluorescent conjugated polyenoic fatty acids as probes of membrane structure is introduced, including phase transitions of phospholipid/cholesterol dispersions, induced circular dichroism of parinaric acid bound to albumin, and biosynthetic incorporation of parinic acid into biological membranes are discussed.

TL;DR: The use of the fluorescent fatty acid, parinaric acid (9, 11, 13, 15-octadecatetraenoic acid) (PnA), was studied in cells of an unsaturated fatty acid auxotroph of Escherichia coli and significant quenching of tryptophan fluorescence by PnA was observed.