Lawrence W. Zettler

TL;DR: Propagation of the auto-pollinated terrestrial orchid, Platanthera clavellata, using symbiotic seed germination using mycorrhizal fungi in vitro is described and the ecological role of this orchid with its associated fungi is discussed with respect to the other co-habiting Platanthersa species.

TL;DR: The symbiotic technique to germinate seeds of three semi-aquatic rein orchid species from Florida and to cultivate H. repens seedlings has the potential to be adopted by wetland restoration projects seeking to include an orchid and mycorrhizal fungi as biotic agents.

TL;DR: Germination is considerably enhanced with mycorrhizal fungi that facilitate the absorption of free water by their orchid seed hosts, implying that epiphytic orchids are capable of germinating quickly given an adequately moist substrate.

TL;DR: Symbiotic germination was evaluated to determine need for cold moist stratification to enhance seed germination and seedling development and identify the mycobionts that support in vitro Germination and development and results indicate that in vitro development of P. praeclara is best supported when stratified seeds are cultured with fungi isolated from young seedlings.

TL;DR: A technique to germinate seeds and cultivate seedlings of P. leucophaea in vitro using cold treatments and mycorrhizal fungi to make it possible for this threatened orchid to be propagated in soil ex vitro, followed by reintroduction into suitable habitats.