Ligia Morganti

TL;DR: Applications in protein processing are described and variations of immobilized metal ion affinity chromatography are described as well as other metal affinity-based technologies.

TL;DR: A six-step, high-yield purification procedure for the preparation of clinical grade recombinant human growth hormone secreted in bacterial periplasmic space is described, with particular emphasis on hormone recovery yields and maximum contaminant host cell elimination.

TL;DR: It is demonstrated that high pressure can successfully convert insoluble IBs of ES expressed in Escherichia coli into an ES preparation with native tertiary structure and full biological activity.

TL;DR: It is shown that long term induction of expression in E. coli enabled the recovery of VapC in inclusion bodies and suggests that the cleavage of the initiator transfer RNA may represent a common mechanism to a larger group of bacteria and potentially configuration a mechanism of post-transcriptional regulation leading to the inhibition of global translation.

TL;DR: Human thyroid‐stimulating hormone (hTSH), a heterodimeric glycoprotein composed of non‐covalently linked α‐ and β‐subunits, was expressed in Chinese hamster ovary cells using a system based on dicistronic expression vectors.