The invention relates to an Aureobasidium pullulans strain and an application thereof, and belongs to the technical fields of fermentation engineering and biology The preservation name of the Aureobasidium pullulans strain is Aureobasidium pullulans ZK-502, the strain is preserved in CGMCC (China General Microbiological Culture Collection Center) on August 20, 2018 at No3 of No1 West Beichen Road, Chaoyang District, Beijing, and the preservation number is CGMCC No 16326 The Aureobasidium pullulans strain can efficiently produce liamocin by fermentation and produces no melanin, fermentation broth is light pink to faint yellow, and follow-up treatment process can be simplified The method is simple and feasible, raw materials are simple in composition, yield of liamocin can reach 8-10 g/L, and commercial and large-scale production of liamocin is expected to be realized; the obtained liamocin product is dissoluble in water and has wide application prospect in medicine, cosmetics andfood

Aureobasidium pullulans strain and application thereof

The invention relates to a streptomyces albus X-18 and method for producing epsilon-polylysine by using the same, and belongs to the technical field of bioengineering. The streptomyces albus X-18 is separated and screened from soil and is preserved, and the preservation number is CGMCC No.17536. The streptomyces albus X-18 is used for producing the epsilon-polylysine, a fermentation culture mediumand fermentation conditions are improved, and intermediate material supplementation and a method for adding inducers are also used so that the fermentation yield of the epsilon-polylysine can reach 3.5-4.2 g/L. A strain with the independent intelligent property right is mainly used, and corn starch low in price is used as the main component of the fermentation culture medium, so that the production cost is reduced, and the fermentation yield is improved.

Streptomyces albus X-18 and method for producing epsilon-polylysine by using same

The invention belongs to the field of tea cream, and particularly relates to a tea cream with eurotium cristatum and mulberry leaves and a preparation method of the tea cream. The preparation method comprises the following steps: culturing eurotium cristatum liquid at first; inoculating on the mulberry leaves; performing wet fermentation and culturing for 5-10 days until the eurotium cristatum grows vigorously; drying in shade and continuing to place and age for half a month to obtain wet eurotium cristatum-fermented mulberry leaves; soaking, pressing and filtering to obtain Fuzhuan tea mulberry leaf juice; and finally, concentrating to prepare the cream by low-temperature concentration and other processes. Wet fermentation conditions provided by the invention can better control the growthof eurotium cristatum and culture better fungi, thereby directly preparing cream and powder; and the preparation method provided by the invention integrates cream preparation with fermentation and improves a medicinal value of the mulberry leaves.

Tea cream with eurotium cristatum and mulberry leaves and preparation method of tea cream

The invention belongs to the field of deep processing of aquatic products, and relates to a preparation method for polysaccharide from a cooking liquor of clams or scallops. The method provided by theinvention obtains a milk-white powdery polysaccharide finished product from the cooking liquor processed from the clams or the scallops by using the cooking liquor processed from the clams or the scallops as a raw material through the steps of raw material concentration, centrifuging for removal of impurities, ultrafiltration, ethanol precipitation, enzymolysis, secondary ethanol precipitation, freeze-drying, etc. The recovery rate of the polysaccharide reaches 70%; and the purity of the polysaccharide is 80% or more. The method provided by the invention has the advantages of simple process,greenness, environmental friendliness, high polysaccharide yield and purity, applicability to industrial amplification, simple steps, convenient operation and strong practicability.

Preparation method for polysaccharides from cooking liquor of clams or scallops

The invention provides an improved method for preparing mussel oligosaccharides with yeast. The method comprises the following steps: (1) providing yeast capable of secreting amylase; (2) culturing the yeast of the step (1) with mussel polysaccharides; and (3) after the culture is completed, removing the yeast and undegraded mussel polysaccharides and proteins to obtain the mussel oligosaccharides. Compared with the original process, the method of the invention can shorten time, reduce cost and improve yield.

Improved method for preparing mussel oligosaccharides with yeast

The invention relates to an epsilon-polylysine high-yielding strain and an epsilon-polylysine production method, and belongs to the biotechnical field. The epsilon-polylysine high-yielding strain EA-19 is named as Streptomyces albus, and the preservation number is CGMCC No.10156. The strain EA-19 is used to carry out fed batch fermentation culture, and the concentration of epsilon-polylysine in a fermentation liquid reaches 38.5-39 g/L. The epsilon-polylysine production method comprises is characterized in that the strain EA-19 is adopted to carry out fermentation culture. A bubbling technology is adopted, ventilation is carried out in and after the fermentation culture process, so epsilon-polylysine generated after fermentation exists in foams, and is separated from the fermentation liquid, so the technical problem of difficulty separation of epsilon-polylysine in present preparation methods is solved, a step of ion exchange which causes generation of a large amount of wastewater is omitted, and the product yield is improved by 50% or above. The method has the advantages of simple process, easiness in amplification, low energy consumption, small device investment, and easiness in realization of large-scale production.

Epsilon-polylysine high-yielding strain and epsilon-polylysine production method

The invention belongs to the technical field of tea leave processing and particularly relates to a production method of concentrated tea liquor. The production method includes the steps of firstly, fermenting, to be more specific, adding raw material tea into a fermentation tank, sterilizing, cooling, adding water, inoculating with Eurotium cristatum inoculation liquid, and performing solid-liquid fermentation; secondly, performing solid-liquid separation, to be more specific, performing primary solid-liquid separation on fermented liquid to remove tea residues, and using a ceramic film to finely filter primary filtrate to obtain tea extract; thirdly, concentrating the tea extract to obtain the concentrated tea liquor; fourthly, sterilizing the concentrated tea liquor to obtain the concentrated tea liquor product. The production method has the advantages that tea produced by various production processes such as a dark tea production process, a black tea production process, a green tea production process and a white tea production process, and the production method is simple in process, capable of lowering tea fragrance loss, effective component loss and characteristic flavor substance reduction during concentrated tea liquor production and capable of producing the concentrated tea liquor product smooth and mellow in taste.

Production method of concentrated tea liquor

The invention belongs to the field of aquatic product deep processing, and especially relates to an industrial extraction method of mussel polysaccharide. According to the method, frozen cooked dried mussels are taken as the raw materials, the preparation method comprises steps of raw material pretreatment, extraction, dreg removal, enzymatic hydrolysis, filtration, ethanol precipitation, dehydration, filtration, and drying, the finished product of mussel polysaccharide is white powder, the yield of polysaccharide is 7.9 to 12.4%, and the content of mussel polysaccharide is 82 to 92%. The provided method has the advantages that the technology is simple, green, and environment-friendly, the polysaccharide yield is high, the purity is high, the method is suitable for industrialized enlargement, the steps are simple, the operation is convenient, and the practicality is strong.

Industrial extraction method of mussel polysaccharide

The invention relates to a hop yeast tabletting candy and a preparation method thereof The hop yeast tabletting candy is prepared from the raw materials in parts by mass: 15 to 20 parts of hop, 10 to 18 parts of chromium-riched yeast, 75 to 80 parts of filler, 1 to 2 parts of lubricating agent, and 5 to 10 parts of adhesion agent The preparation method comprises the steps of adopting the hop and the chromium-riched yeast as main functional components, mixing with the filler and the adhesion agent in proportion, using a boiling dryer for pelletizing, and then adding the lubricating agent for tabletting into tablets The preparation method provided by the invention adopts a one-step pelletizing method, so that the production efficiency is high The prepared tabletting candy has delicate taste and pleasant hop aroma and yeast aroma, is convenient to carry and eat, can be used for assisting to reduce blood glucose of a diabetic patient, reduce serum cholesterol and improve endocrine, has a remarkable prevention and curative effect on prostatitis, prostate cancer and other prostate diseases, and further has a certain adjuvant therapy efficacy on diseases such as breast cancer and colon cancer at the same time The preparation method is simple in steps, convenient to operate, and high in practicability

Hop yeast tabletting candy and preparation method thereof

The invention discloses aureobasidium pullulan alb1 gene knockout mutant strain. According to the mutant strain, all or part of coding genes in alb1 gene in aureobasidium pullulan strain are knocked out and replaced by a hygromycin B resistance gene cassette. The hygromycin B resistance gene cassette comprises aureobasidium pullulan transcription elongation factor promoter PTEF and hygromycin B resistance gene HrgR; the sequence of the aureobasidium pullulan transcription elongation factor promoter PTEF is as shown in SEQ ID NO.2 in a sequence table; the sequence of the hygromycin B resistance gene HrgR is as shown in SEQ ID NO.3 in the sequence table. The mutant strain is used for the fermentation production of microbial polysaccharide, does not produce melanin, and can obviously the yield and quality of polysaccharide during the purification process.

Liu Fei

Papers

Epsilon-polylysine high-yielding strain and epsilon-polylysine production method

Production method of concentrated tea liquor

Industrial extraction method of mussel polysaccharide

Hop yeast tabletting candy and preparation method thereof

Aureobasidium pullulan alb1 gene knockout mutant strain and application thereof