Showing papers by "Luis Hernandez published in 1983"
TL;DR: A new technique for in vivo neurotransmitter extraction was developed by chronically implanted in the caudate or the thalamus of rats and measurable amounts of dopamine and norepinephrine were recovered from the perfusion fluid.
Abstract: A new technique for in vivo neurotransmitter extraction was developed. Perfusion U shaped cannulae were chronically implanted in the caudate or the thalamus of rats. A segment of the cannula was a piece of dialysis tube of molecular weight cut off 1000. Measurable amounts of dopamine and norepinephrine were recovered from the perfusion fluid. Intraperitoneal amphetamine 10 mg/kg increased dopamine output from the caudate but not from the thalamus. The potential applications of this technique are discussed.
44 citations
Journal Article•
TL;DR: A brief period of anorexia was followed by hyperphagia and chronic obesity, suggesting that amphetamine acts in the lateral hypothalamus not only to suppress feeding, but in high doses it may also have local neurotoxic effects that cause an upward shift in body weight maintained by overeating.
Abstract: Overeating and obesity relative to controls was produced by multiple bilateral injections of 0.5 mg of amphetamine in the lateral ventricles of female rats eating a palatable, high fat diet. This behavioral and physiological rebound following the expected period of anorexia was accompanied by long-term depletion of dopamine in the striatum and of norepinephrine in the hypothalamus. This suggested the next experiment in which 50 micrograms of amphetamine were injected repeatedly in the lateral hypothalamus; again a brief period of anorexia was followed by hyperphagia and chronic obesity. This suggests that amphetamine acts in the lateral hypothalamus not only to suppress feeding, but in high doses it may also have local neurotoxic effects that cause an upward shift in body weight maintained by overeating.
13 citations