Showing papers by "Luis M. Montuenga published in 2003"
TL;DR: The results suggest that the expression and processing of each hnRNP protein in lung cancer is independently regulated and is not exclusively related to proliferation status.
152 citations
TL;DR: The morphological, histochemical and functional traits of these cells in three major systems gastrointestinal, respiratory and prostatic DES are described, focusing on some aspects of their ontogeny and differentiation, as well as to their relevance in carcinogenesis, especially in neuroendocrine tumors.
48 citations
TL;DR: Simultaneous exposure to LPS and hypoxia results in a greater influx of inflammatory cells that further enhances NOS2 expression in rat lungs through the recruitment of N OS2-producing leukocytes.
Abstract: The potential role of nitric oxide synthase-2 (NOS2) in acute lung injury (ALI) has gained increasing attention. This study evaluates the effects of hypoxia, an important feature of ALI, on NOS2 expression in a rat model of ALI caused by exposure to hypoxia and LPS. Exposure to hypoxia alone had no effect on the expression of NOS2 in rat lungs. LPS treatment resulted in a significant increase in NOS2 in the lungs, which was further enhanced by concomitant exposure to hypoxia. Immunohistochemical analysis and in situ hybridization showed no changes in the expression of NOS2 in lung resident cells under any conditions. The increase in NOS2 levels is mainly due to the influx of NOS2-expressing inflammatory cells. By morphologic analysis, these inflammatory cells were identified as neutrophils, lymphocytes, and monocytes. In vitro experiments of lung epithelial and endothelial cell lines showed no detectable expression of NOS2 with any of the treatments. In a macrophage cell line, LPS-induced NOS2 expression was not affected by the concomitant exposure to hypoxia. In conclusion, LPS increases NOS2 expression in rat lungs through the recruitment of NOS2-producing leukocytes. Simultaneous exposure to LPS and hypoxia results in a greater influx of inflammatory cells that further enhances NOS2 expression.
48 citations
TL;DR: It is demonstrated that preproAM and PAM expression in the human prostate is androgen‐independent and for the first time the expression of a novel PAM transcript in PC is reported, which has not been previously described in other tissues.
Abstract: Most of the locally advanced and metastatic prostate carcinomas (PCs) treated with antiandrogenic therapy eventually become refractory to this treatment. Locally produced factors may control prostate tumor biology after androgen withdrawal. Adrenomedullin (AM) is expressed in the prostate and could control cell growth in androgen-independent conditions. AM needs to be amidated by the enzyme peptidylglycine alpha-amidating monooxygenase (PAM) to become fully active. The objective of the present study was to analyze whether the expression of preproadrenomedullin (preproAM) and PAM in PC is regulated by androgens. For this purpose, human in vitro and in vivo PC models were grown in the presence or absence of androgens, and the expression of AM and PAM was examined by immunohistochemistry, Western blotting, RT-PCR, and Northern blotting. Furthermore, immunohistochemical analysis of AM in clinical specimens was performed to test if its expression is related to Gleason score and antiandrogenic therapy. In PC cell lines and xenografts, mRNA and protein AM levels were similar in the presence or absence of androgens. PAM expression seemed to be induced by androgen-withdrawal. Our results in clinical samples showed no relationship between AM expression and Gleason score or antiandrogenic treatment. In conclusion, our results demonstrate that preproAM and PAM expression in the human prostate is androgen-independent. In addition, we also report for the first time the expression of a novel PAM transcript in PC, which has not been previously described in other tissues.
22 citations
TL;DR: In vivo and in vitro studies suggest that AM could strongly suppress the malignancy of prostate cancer cells, via autocrine and/or paracrine mechanisms.
21 citations
TL;DR: Siguiendo el protocolo descrito, es posible detectar el cancer de pulmon en estadios tempranos en sujetos asintomaticos with factores of riesgo para desarrollar esta enfermedad.
Abstract: Fundamento y objetivo: Presentar los datos iniciales de un programa de deteccion precoz del cancer de pulmon por tomografia computarizada de baja dosis de radiacion, asi como exponer los criterios de inclusion y el algoritmo de actuacion inicial de este proyecto. Sujetos y metodo: Desde septiembre de 2000 a mayo de 2001 se realizo una TC toracica con baja dosis de radiacion a 150 fumadores asintomaticos (intervalo de edad, 40-78 anos). El protocolo de estudio incluia: TC basal de baja dosis de radiacion, control evolutivo por TC convencional de los nodulos pulmonares no calcificados de diametro inferior a 10 mm y exploraciones complementarias (tomografia por emision de positrones [PET]) de los nodulos mayores de 10 mm de diametro, potencialmente malignos. Resultados: Se detectaron 54 nodulos pulmonares no calcificados en 34 sujetos asintomaticos (24,15%). El diametro mayor fue de 5 mm o menos en 24 individuos (70,6%), entre 6 y 10 mm en 7 (20,6%) y mayor de 10 mm en tres (8,8%). Se realizo una PET en un unico caso, con resultado positivo y diagnostico histologico de carcinoma escamoso. Los otros dos nodulos no calcificados mayores de 10 mm se consideraron probablemente benignos al presentar un aspecto radiologico de lesion cicatrizal residual. Conclusion: Siguiendo el protocolo descrito, es posible detectar el cancer de pulmon en estadios tempranos en sujetos asintomaticos con factores de riesgo para desarrollar esta enfermedad. Aunque los resultados preliminares son esperanzadores, es preciso incrementar el tamano de la muestra antes de obtener conclusiones definitivas.
3 citations