Showing papers by "Lusânia Maria Greggi Antunes published in 1999"
TL;DR: Evidence has been accumulated indicating that a diet rich in antioxidants reduce the risks of the major human diseases, and the importance of dietary antioxidants in the defense strategies of organisms against free radicals is discussed.
Abstract: Durante a reducao do oxigenio molecular, especies reativas de oxigenio sao formadas e existe a necessidade permanente de inativar estes radicais livres. Os danos induzidos pelos radicais livres podem afetar muitas moleculas biologicas, incluindo os lipideos, as proteinas, os carboidratos e as vitaminas presentes nos alimentos. As especies reativas de oxigenio tambem estao implicadas nas varias doencas humanas. Evidencias tem sido acumuladas indicando que uma dieta rica em antioxidantes reduz os riscos das principais doencas humanas. Esta revisao discute a importância dos antioxidantes da dieta sobre as estrategias de defesa dos organismos contra os radicais livres.
219 citations
TL;DR: Investigation of the effects of VC on human peripheral blood lymphocytes in vitro and its anticlastogenic effect on chromosomal aberrations induced by doxorubicin found the efficiency of VC in protecting against chromosome damage was dependent on the doses used.
Abstract: Some chemotherapeutic approaches have proposed the use of antioxidants such as vitamin C (VC) to minimize the cytotoxicity and damage induced in normal tissue by antitumor agents that produce free radicals. Nevertheless, VC can also be cytotoxic, genotoxic, and harmful when combined with antitumor agents in human cells in vitro. The present study was undertaken to investigate the effects of VC (100, 200, 500, and 1,000 μg/ml) on human peripheral blood lymphocytes in vitro and its anticlastogenic effect on chromosomal aberrations induced by doxorubicin (DXR). VC did not show a clastogenic effect by itself, except at 1,000 μg/ml. At the concentration of 100 or 200 μg/ml of VC, administered in pre-, post-, or simultaneous treatment, there was a significant reduction in both chromosome aberrations and number of abnormal metaphases induced by DXR. At the doses of 500 or 1,000 μg/ml, VC did not present the same protective effect and was cytotoxic. Under the present experimental conditions, the efficiency of VC in protecting against chromosome damage was dependent on the doses used. Teratogenesis Carcinog. Mutagen. 19:53–59, 1999. © 1999 Wiley-Liss, Inc.
41 citations
TL;DR: A statistically significant increase in the frequency of chromosomal damage was observed when the middle and the highest concentrations of curcumin were associated with DXR during the G1/S, S, and S/G2 phases of the cell cycle.
Abstract: Curcumin, a natural phenolic compound, is gaining importance as a free radical scavenger. This study was undertaken to investigate the modulatory effects of curcumin on the chromosomal damage induced by the antitumoral doxorubicin (DXR), a known free radical generator, in Chinese hamster ovary cells in culture. Cells were treated with three concentrations of curcumin (2.5, 5, or 10 microg/ml) and then treated with DXR (1.0 microg/ml) during different phases of the cell cycle. The results show that curcumin induces chromosomal damage in CHO at the highest concentration when compared to the untreated control. Neither treatment with curcumin shows a reduction in the clastogenicity of DXR. Instead, a statistically significant increase in the frequency of chromosomal damage was observed when the middle and the highest concentrations of curcumin were associated with DXR during the G1/S, S, and S/G2 phases of the cell cycle. The results clearly indicate the potentiating effect of curcumin on DXR-induced chromosomal damage.
36 citations
TL;DR: Os resultados obtidos mostraram that a VC foi efetiva na reducao estatisticamente significativa da clastogenicidade da DDP em ratos Wistar (aproximadamente 70%).
Abstract: The ability of vitamin C (VC) to protect against the clastogenic action of the chemotherapeutic agent cisplatin (DDP, cis-diamminedichloroplatinun II) in rat bone marrow cells was evaluated. DDP was administered to Wistar rats either alone or after treatment with VC. The rats were treated with VC (50, 100 or 200 mg/kg body weight) by gavage 10 min before the administration of DDP (5 mg/kg body weight, ip) and then sacrificed 24 h after treatment. VC significantly reduced (by about 70%) the clastogenicity of DDP in rat bone marrow cells. The antioxidant action of VC presumably modulates the clastogenic action of DDP.
24 citations
TL;DR: Rat bone marrow cells treated simultaneously with olive oil and Doxorubicin developed significantly fewer chromosomal aberrations and abnormal metaphases than those treated with DXR alone.
Abstract: There is considerable interest in identifying dietary compounds which have the capacity to protect against chromosomal aberrations induced by antitumor agents. Fatty acids and their constituents are able to act as free radical scavengers. Doxorubicin (DXR) is an important chemotherapeutic agent, that also induces chromosome aberrations. Rat bone marrow cells treated simultaneously with olive oil (10 ml/kg body weight) and DXR (90 mg/kg body weight) developed significantly fewer chromosomal aberrations and abnormal metaphases than those treated with DXR alone.
12 citations