M
Makio Tokunaga
Researcher at Tokyo Institute of Technology
Publications - 66
Citations - 6967
Makio Tokunaga is an academic researcher from Tokyo Institute of Technology. The author has contributed to research in topics: Myosin & Myosin head. The author has an hindex of 28, co-authored 62 publications receiving 6479 citations. Previous affiliations of Makio Tokunaga include Olympus Corporation & National Institute of Genetics.
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Highly inclined thin illumination enables clear single-molecule imaging in cells.
TL;DR: A simple illumination method of fluorescence microscopy for molecular imaging yielded clear single-molecule images and three-dimensional images using cultured mammalian cells, enabling one to visualize and quantify molecular dynamics, interactions and kinetics in cells for molecular systems biology.
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Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution
TL;DR: This approach can be used directly to image single fluorescently labelled myosin molecules and detect individual ATP turnover reactions and can be applied to the study of many types of enzymes and biomolecules.
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Newly generated T cell receptor microclusters initiate and sustain T cell activation by recruitment of Zap70 and SLP-76.
Tadashi Yokosuka,Kumiko Sakata-Sogawa,Wakana Kobayashi,Michio Hiroshima,Akiko Hashimoto-Tane,Makio Tokunaga,Makio Tokunaga,Michael L. Dustin,Takashi Saito +8 more
TL;DR: It is reported here that T cell activation was initiated and sustained in TCR-containing microclusters generated at the initial contact sites and the periphery of the mature immunological synapse.
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Zinc is a novel intracellular second messenger
Satoru Yamasaki,Kumiko Sakata-Sogawa,Aiko Hasegawa,Tomoyuki Suzuki,Koki Kabu,Emi Sato,Tomohiro Kurosaki,Susumu Yamashita,Makio Tokunaga,Makio Tokunaga,Keigo Nishida,Toshio Hirano +11 more
TL;DR: The results suggest that the zinc wave is involved in intracellular signaling events, at least in part by modulating the duration and strength of FcɛRI-mediated signaling.
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A single myosin head moves along an actin filament with regular steps of 5.3 nanometres
TL;DR: A new instrument is developed with which individual myosin subfragment-1 molecules are captured and directly manipulated using a scanning probe to resolve the individual mechanical events of force generation by actomyosin.