Manfred Schrewe

TL;DR: This review focuses on highlighting the potentials, limitations, and solutions offered by the application of self-regenerating microbial cells as biocatalysts, especially in C-O functional group chemistry.

TL;DR: AlkL is an efficient tool to boost productivities of whole-cell biotransformations involving hydrophobic aliphatic substrates and thus has potential for broad applicability, and clear evidence for a prominent role of AlkL in alkane degradation is shown.

TL;DR: The presented proof of concept shows that heterologous pathway engineering allowed the construction of a whole-cell biocatalyst catalyzing the terminal amino-functionalization of fatty acid methyl esters and alkanes to demonstrate the tremendous potential of whole- cell bioc atalysts for the production of industrially relevant building blocks.

TL;DR: The versatility of whole‐cell biocatalysis for synthesis of industrially relevant bifunctional building blocks is highlighted and how integrated reaction and catalyst engineering can be implemented to control product formation patterns inBiocatalytic multistep reactions is demonstrated.

TL;DR: The efficient coupling of redox cofactor oxidation and product formation, as determined in vitro, combined with the high in vivo activities make E. coli W3110 (pBT10) a promising biocatalyst for the preparative synthesis of terminally functionalized fatty acid methyl esters.