Showing papers by "María Teresa Camacho Olmedo published in 2015"
TL;DR: How to compare the outputs from models that simulate transitions among categories through time and how to compare output maps from pairs of model runs with respect to a reference map of transitions during the validation interval is illustrated.
Abstract: Our article illustrates how to compare the outputs from models that simulate transitions among categories through time. We illustrate the concepts by comparing two land change models: Land Change Modeler and Cellular Automata Markov. We show how the modeling options influence the quantity and allocation of simulated transitions, and how to compare output maps from pairs of model runs with respect to a reference map of transitions during the validation interval. We recommend that the first step is to assess the quantity of each transition and to determine the cause of the variation in quantity among model runs. The second step is to assess the allocation of transitions and to determine the cause of the variation in allocation among model runs. The separation of quantity and allocation of the transitions is a helpful approach to communicate how models work and to describe pattern validation. We compare three runs of models that simulate transitions among land categories.Pattern validation compares a reference map of transition to maps from pairs of runs.Quantity and allocation are helpful concepts to describe models and to compare maps.Quantity refers to the size of each transition from one category to another category.Allocation refers to the spatial distribution of the transitions.
104 citations
TL;DR: An automated method to monitor the timing of these discrete phases of C. elegans maturation, from the first larval stage through adulthood, using bioluminescence is described, which will allow dissecting the molecular machinery governing the speed of the developmental clock, which has so far been hampered by the lack of a method suitable for genetic screens.
Abstract: Caenorhabditis elegans postembryonic development consists of four discrete larval stages separated by molts. Typically, the speed of progression through these larval stages is investigated by visual inspection of the molting process. Here, we describe an automated method to monitor the timing of these discrete phases of C. elegans maturation, from the first larval stage through adulthood, using bioluminescence. The method was validated with a lin-42 mutant strain that shows delayed development relative to wild-type animals and with a daf-2 mutant that shows an extended second larval stage. This new method is inherently high-throughput and will finally allow dissecting the molecular machinery governing the speed of the developmental clock, which has so far been hampered by the lack of a method suitable for genetic screens.
47 citations
TL;DR: It is concluded that entrainment can be utilized to discover hitherto unknown circadian clocks and the implications of using entrained phase angles more broadly, even in model systems that show robust free-running rhythms.
Abstract: Three properties are most often attributed to the circadian clock: a ca. 24-h free-running rhythm, temperature compensation of the circadian rhythm, and its entrainment to zeitgeber cycles. Relatively few experiments, however, are performed under entrainment conditions. Rather, most chronobiology protocols concern constant conditions. We have turned this paradigm around and used entrainment to study the circadian clock in organisms where a free-running rhythm is weak or lacking. We describe two examples therein: Caenorhabditis elegans and Saccharomyces cerevisiae. By probing the system with zeitgeber cycles that have various structures and amplitudes, we can demonstrate the establishment of systematic entrained phase angles in these organisms. We conclude that entrainment can be utilized to discover hitherto unknown circadian clocks and we discuss the implications of using entrainment more broadly, even in model systems that show robust free-running rhythms.
6 citations