Mariana L. Fazenda

TL;DR: The Pichia pastoris expression system is being used successfully for the production of various recombinant heterologous proteins and the importance of optimizing the physicochemical environment for efficient and maximal recombinant protein production in bioreactors and the role of process control in optimizing protein production is reviewed.

TL;DR: This chapter evaluates recent advances in submerged liquid cultivation of the vegetative or mycelial forms of those members of the “higher fungi” that produce macroscopic spore-bearing structures, namely the macrofungi, those producing potentially useful natural products and novel compounds with interesting biological activities by means of submerged culture fermentation processes.

TL;DR: This review uses pullulan, curdlan, xanthan, and fungal β-glucans as examples of industrially produced microbial exopolysaccharides, and the role of fermenter configuration in their production is examined.

TL;DR: Morphologically, dispersed mycelium was predominant under controlled DO conditions, with highly branched hyphae, consistent with the enhanced culture growth noted under these conditions, and rheological terms, broths demonstrated shear-thinning behaviour with a yield stress under both DO conditions.

TL;DR: The findings of this study show for the first time the potential of NIRS as an input generating for MFA models, contributing to the optimization of cell factory metabolism in real-time.