Showing papers by "Marilyn C. Roberts published in 1991"
TL;DR: Mobiluncus, Gardnerella vaginalis and Mycoplasma hominis were independently associated with a clinical diagnosis of bacterial vaginosis, and Mobiluncus was further associated with the presence of BV-associated microorganisms (M hominis and G vaginalis), N gonorrhoeae, black race, and gravidity.
Abstract: The microbiological and epidemiological correlates of vaginal colonisation by Mobiluncus species were examined among randomly selected women attending a sexually transmitted disease (STD) clinic. Women positive for Trichomonas vaginalis were excluded. Mobiluncus spp. were detected by Gram stained vaginal smear in 21% of 633 STD clinic patients, including 53% of those with and 4% of those without bacterial vaginosis (BV), as diagnosed by clinical criteria. Gardnerella vaginalis and Mycoplasma hominis detected by vaginal culture and Mobiluncus detected by vaginal Gram stain were each independently associated with BV after adjusting by logistic regression for the presence of sexually transmitted disease pathogens, gravidity, parity and number of lifetime sexual partners (p less than 0.001 for each organism). Bacterial vaginosis was negatively correlated with isolation of lactobacilli, yeast and herpes simplex virus. After adjusting for presence or absence of BV, women with Mobiluncus were more likely to harbour G vaginalis (odds ratio 5.6, 95% confidence interval 1.6-19.5), M hominis (OR 3.7, 95% CI 2.0-7.0) and Neisseria gonorrhoeae (OR 2.9, 95% CI 1.4-6.0) and less likely to harbour vaginal yeast (OR 0.4, 95% CI 0.2-1.0); were more likely to be black (OR 2.7, 95% CI 1.5-4.6), and to have been pregnant (OR 1.8, 95% CI 1.1-3.1); but after the adjustment for BV, vaginal colonisation by Mobiluncus was not associated with symptoms of odour, abdominal pain, menstrual irregularities, or with adnexal tenderness. In summary, Mobiluncus, Gardnerella vaginalis and Mycoplasma hominis were independently associated with a clinical diagnosis of bacterial vaginosis, and Mobiluncus was further associated with the presence of BV-associated microorganisms (M hominis and G vaginalis), N gonorrhoeae, black race, and gravidity.
34 citations
TL;DR: Seventy-one streptococci isolated from dairy cows with clinical mastitis were tested for tetracycline resistance, suggesting that at least two different uncharacterized Tet determinants exist in these isolates, one conferring high resistance to both tetrACYcline and minocycline
28 citations
TL;DR: Two tetracycline-resistant Moraxella (Branhamella) catarrhalis strains from England were compared with two previously characterized Tcr Texas strains, which demonstrated that the strains from the same area were identical (clonal); however, the Texas and English strains differed from each other.
Abstract: Two tetracycline-resistant (Tcr) Moraxella (Branhamella) catarrhalis strains from England were compared with two previously characterized Tcr Texas strains. Both pairs carried the Tet B determinant, which was nontransferable. Pulsed-field gel electrophoresis of their genomic DNA restriction fragments demonstrated that the strains from the same area were identical (clonal); however, the Texas and English strains differed from each other.
23 citations
TL;DR: Tetracycline-resistant Tet M-negative isolates of Actinomyces viscosus, Eubacterium lentum, Mobiluncus curtisii, and Mobil uncus mulieris were screened with the Tet K, Tet L, and Tet O DNA probes and hybridized with these probes.
Abstract: Tetracycline-resistant Tet M-negative isolates of Actinomyces viscosus, Eubacterium lentum, Mobiluncus curtisii, and Mobiluncus mulieris were screened with the Tet K, Tet L, and Tet O DNA probes. Ten (71%) of the resistant Mobiluncus strains hybridized with the Tet O probe, two of the three E. lentum strains hybridized with the Tet K probe, and the A. viscosus isolate hybridized with the Tet L probe.
19 citations
TL;DR: Of 15 Peptostreptococcus sp.
Abstract: Of 15 Peptostreptococcus sp. strains isolated between 1975 and 1984, 13 hybridized with the Tet K, Tet M, or Tet O determinant. A donor Peptostreptococcus anaerobius strain carrying the three determinants could transfer Tet M to P. anaerobius and Fusobacterium nucleatum recipients but not to an Enterococcus faecalis recipient, while neither Tet K nor Tet O was transferred.
9 citations
TL;DR: The characterization of these atypical isolates has important implications for future investigations in which serological methods are used for diagnosis, epidemiology, and determination of pathogenicity of Mobiluncus spp.
Abstract: Classifications of 48 atypical clinical isolates of Mobiluncus spp. were determined on the basis of biochemical reactions, morphology, antigenic profiles, and DNA hybridization studies. Two new subgroups with unique antigenic profiles are described. Like typical Mobiluncus species, the antigenic variant of M. mulieris is associated with bacterial vaginosis. The atypical isolates of M. curtisii were frequently recovered from women with normal vaginal flora and were also recovered from sterile body sites. These isolates may be incorrectly identified if current biochemical and morphological criteria are used for identification. Gram stain morphology, however, correctly identifies these isolates to the species level. The characterization of these atypical isolates has important implications for future investigations in which serological methods are used for diagnosis, epidemiology, and determination of pathogenicity of Mobiluncus spp.
8 citations
31 Dec 1991
1 citations