Showing papers by "Mark E. Gurney published in 1992"
TL;DR: The sprouting stimulus delivered by the factors showed limited penetrance into the muscle and restricted lateral spread from the injection site, and the effect of CNTF was potentiated twofold by coadministration of bFGF at doses of 2-20 ng daily, whereas treatment with bF GF alone failed to induce sprouting from either end plates or nodes of Ranvier.
Abstract: Ciliary neurotrophic factor (CNTF) and basic fibroblast growth factor (bFGF) were tested for effects on sprouting by motor neurons innervating the adult mouse gluteus muscle. Factors were delivered by subcutaneous injection directly over the surface of the superior gluteus muscle once daily for 7 d and then end plates and axons were visualized by combined silver and cholinesterase staining. CNTF (500 ng daily) induced sprouting both from end plates and from the subset of nodes of Ranvier that are closest to the end plate. The effect of CNTF was potentiated twofold by coadministration of bFGF at doses of 2-20 ng daily, whereas treatment with bFGF alone failed to induce sprouting from either end plates or nodes of Ranvier. The sprouting stimulus delivered by the factors showed limited penetrance into the muscle and restricted lateral spread from the injection site.
176 citations
TL;DR: It has been found that the best results are obtained with messenger RNA purified on an oligo(dT)-cellulose column and subsequently probed with full-length DNA probes synthesized with [α- 32 P] dCTP.
Abstract: Publisher Summary To explore the gene expression of some of these growth factors in human gliomas, it has been found that the best results are obtained with messenger RNA purified on an oligo(dT)-cellulose column and subsequently probed with full-length DNA probes synthesized with [α- 32 P] dCTP. The chapter presents some of the findings with this technique. mRNA encoding interleukin Iβ, interleukin 6, and basic fibroblast growth factor is expressed in substantial amounts in some but not all human gliomas. In a study described in the chapter, only a relatively small amount of interleukin 3 was detected in the two tumor lines and this factor was not detected in any of the glioma specimens from the five patients, yet the production and secretion of interleukin 3 by normal astrocytes measured using a bioassay has been well documented. Interleukin 6 was expressed in fairly large amounts in both tumor lines but was found in small quantities in only two of the glioma specimens.