Marvin M. Pace

TL;DR: The cell culture and nuclear transfer techniques described here should allow the use of genetic modification procedures to produce tissues and organs from cloned pigs with reduced immunogenicity for use in xenotransplantation.

TL;DR: In this paper, a fluorogenic stain consisting of carboxyfluorescin diacetate (CFDA) and the relatively membrane-impermeant nuclear stain propidium iodide (PI) was used to distinguish three distinct populations of spermatozoa.

TL;DR: The data represent the most comprehensive information on cattle derived from nuclear transfer procedures and indicate that this emerging reproductive technology offers unique opportunities to meet critical needs in both human health care and agriculture.

TL;DR: In general, cultured fetal cells performed better with respect to pregnancy initiation and calving than adult cells with the exception of cumulous cells, which produced the highest overall pregnancy and Calving rates.

TL;DR: The present invention relates to cloning technologies as discussed by the authors, in part to immortalized and totipotent cells useful for cloning animals, the embryos produced from these cells using nuclear transfer techniques and materials, methods, and processes for establishing such cells, embryos, and animals.