Journal ArticleDOI
Production of cloned pigs from in vitro systems.
Jeff Betthauser,Erik Forsberg,Monica L. Augenstein,Lynette A. Childs,Kenneth J. Eilertsen,Joellyn M. Enos,Todd M. Forsythe,Paul J. Golueke,Gail L. Jurgella,Richard W. Koppang,Tiffany L. Lesmeister,Kelly S. Mallon,Greg D. Mell,Pavla M. Misica,Marvin M. Pace,Martha Pfister-Genskow,Nikolai S. Strelchenko,Gary R. Voelker,Steven R. Watt,Simon Thompson,Michael D. Bishop +20 more
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TLDR
The cell culture and nuclear transfer techniques described here should allow the use of genetic modification procedures to produce tissues and organs from cloned pigs with reduced immunogenicity for use in xenotransplantation.Abstract:
Here we describe a procedure for cloning pigs by the use of in vitro culture systems. Four healthy male piglets from two litters were born following nuclear transfer of cultured somatic cells and subsequent embryo transfer. The initiation of five additional pregnancies demonstrates the reproducibility of this procedure. Its important features include extended in vitro culture of fetal cells preceding nuclear transfer, as well as in vitro maturation and activation of oocytes and in vitro embryo culture. The cell culture and nuclear transfer techniques described here should allow the use of genetic modification procedures to produce tissues and organs from cloned pigs with reduced immunogenicity for use in xenotransplantation.read more
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Journal ArticleDOI
Production of α-1,3-Galactosyltransferase Knockout Pigs by Nuclear Transfer Cloning
Liangxue Lai,Donna Kolber-Simonds,Kwang-Wook Park,Hee-Tae Cheong,Julia L. Greenstein,Gi-Sun Im,Melissa Samuel,Aaron Bonk,August Rieke,Billy N. Day,Clifton N. Murphy,David B. Carter,Robert J. Hawley,Randall S. Prather +13 more
TL;DR: The production of four live pigs in which one allele of the α-1,3-galactosyltransferase locus has been knocked out is reported, paving the way for xenotransplantation of pigs from clonal fetal fibroblast cell lines.
Journal ArticleDOI
Production of α-1,3-galactosyltransferase null pigs by means of nuclear transfer with fibroblasts bearing loss of heterozygosity mutations
Donna Kolber-Simonds,Liangxue Lai,Steven R. Watt,Maria Denaro,Scott Arn,Monica L. Augenstein,Jeffery M. Betthauser,David B. Carter,Julia L. Greenstein,Yanhong Hao,Gi-Sun Im,Zhonghua Liu,Greg D. Mell,Clifton N. Murphy,Kwang-Wook Park,August Rieke,David J. J. Ryan,David H. Sachs,Erik Forsberg,Randall S. Prather,Robert J. Hawley +20 more
TL;DR: An unexpectedly high rate of spontaneous loss of GGTA1 function was observed, with the vast majority of null cells resulting from loss of the WT allele, and congenital abnormalities observed in the heterozygous NT animals did not reappear in the serially produced null animals.
Journal ArticleDOI
The use of pigs in neuroscience: modeling brain disorders.
Nanna Marie Lind,Anette Moustgaard,Jacob Jelsing,Gábor Vajta,Paul Cumming,Axel Kornerup Hansen +5 more
TL;DR: The present paper focuses on evaluating the potential for modeling symptoms, phenomena or constructs of human brain diseases in pigs, the neuropsychiatric disorders in particular.
Journal ArticleDOI
Successful Piglet Production after Transfer of Blastocysts Produced by a Modified In Vitro System
Kazuhiro Kikuchi,Akira Onishi,Naomi Kashiwazaki,Masaki Iwamoto,Junko Noguchi,Hiroyuki Kaneko,Tomiji Akita,Takashi Nagai +7 more
TL;DR: The results suggest that an excellent piglet production system can be established by using this modified IVP system, which produces high-quality porcine blastocysts and has advantages for the generation of cloned and transgenic pigs.
Journal ArticleDOI
Germ Cell Transplantation in Pigs
TL;DR: Results indicate that germ cell transplantation is feasible in immature pigs, and that porcine transplanted cells are retained in the recipient testis for at least 1 mo, a first step toward successful spermatogonial transplantation in a farm animal species.
References
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Journal ArticleDOI
Viable offspring derived from fetal and adult mammalian cells
TL;DR: The birth of lambs from differentiated fetal and adult cells confirms that differentiation of that cell did not involve the irreversible modification of genetic material required for development to term and reinforces previous speculation that by inducing donor cells to become quiescent it will be possible to obtain normal development from a wide variety of differentiated cells.
Journal ArticleDOI
Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei
Teruhiko Wakayama,Anthony C.F. Perry,Anthony C.F. Perry,Maurizio Zuccotti,Maurizio Zuccotti,K R Johnson,Ryuzo Yanagimachi +6 more
TL;DR: These experiments show that for mammals, nuclei from terminally differentiated, adult somatic cells of known phenotype introduced into enucleated oocytes are capable of supporting full development.
Journal ArticleDOI
Sheep cloned by nuclear transfer from a cultured cell line
TL;DR: This is the first report, to the authors' knowledge, of live mammalian offspring following nuclear transfer from an established cell line, and will provide the same powerful opportunities for analysis and modification of gene function in livestock species that are available in the mouse through the use of embryonic stem cells.
Journal ArticleDOI
Cloned transgenic calves produced from nonquiescent fetal fibroblasts
Jose B. Cibelli,Jose B. Cibelli,Steve L. Stice,Steve L. Stice,Paul Golueke,Paul Golueke,J.J. Kane,J.J. Kane,Joseph D. Jerry,Joseph D. Jerry,Cathy Blackwell,Cathy Blackwell,F. Abel Ponce de Leon,F. Abel Ponce de Leon,James M. Robl,James M. Robl +15 more
TL;DR: The life-span of near senescent fibroblasts could be extended by nuclear transfer, as indicated by population doublings in fibroblast lines derived from a 40-day-old fetal clone.
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