Applications of NMR Spectroscopy in Organic Chemistry.

The covalent binding of the hepatocarcinogen aflatoxin B1 by rat liver microsomes to calf thymus DNA resulted in a binding level equal to one aflatoxin residue per 60 DNA nucleotides. An aflatoxin derivative-guanine adduct was efficiently liberated from DNA with formic acid. Analytical reversed-phase high-pressure liquid chromatography of the DNA hydrolysate revealed that approximately 90% of the carcinogen bound to DNA could be accounted for as a single component. Preparative high-pressure liquid chromatography was used to isolate sufficient quantities of the adduct for structural analysis from large quantities (340 mg) of DNA. A combination of spectral and chemical data indicates that the major product of the interaction of metabolically activated aflatoxin B1 and DNA is 2,3-dihydro-2-(N7-guanyl)-3-hydroxyaflatoxin B1 with the guanine and hydroxyl functions possessing a trans configuration. The structural data support the hypothesis that the putative 2,3-oxide of aflatoxin B1 is quantitatively important as an intermediate in the binding of aflatoxin B1 to nucleic acids.

https://www.pnas.org/content/pnas/74/5/1870.full.pdf

Structural identification of the major DNA adduct formed by aflatoxin B1 in vitro

Chemistry and Biology of Mycotoxins and Related Fungal Metabolites

Jin-Ming Gao,*,† Sheng-Xiang Yang,‡ and Jian-Chun Qin †Shaanxi Engineering Center of Bioresource Chemistry & Sustainable Utilization, Department of Chemistry and Chemical Engineering, College of Science, Northwest A&F University, 3 Taicheng Road, Yangling 712100, Shaanxi, China ‡College of Science, Zhejiang A&F University, Lin’an, Hangzhou 311300, Zhejiang, China College of Plant Sciences, Jilin University, Changchun, Jilin 130062, China

Azaphilones: Chemistry and Biology

The invention relates to ophthalmological compositions for topical treatment of glaucoma or ocular hypertension comprising an effective intraocular pressure reducing amount of a prostaglandin derivative of PGA, PGB, PGD, PGE or PGF, in which the omega chain contains a ring structure, in an ophthalmologically compatible carrier. The invention further relates to the preparation of said compositions and their use for treatment of glaucoma or ocular hypertension.

Prostaglandin derivatives for the treatment of glaucoma or ocular hypertension

Total synthesis of racemic aflatoxin B1

The biosyntheses of monascorubrin and monascoflavin by a strain of Monascus genus have been studied and the two pigments have shown to be derived from the linear condensation of acetic acid units. The results confirm the proposed structure of both pigments.

Biosyntheses of Monascorubrin and Monascoflavin.

Nature of photochemically induced transannular hydrogen abstractions of taxinines

A process for separating and recovering serum and plasma components from a whole blood sample including passing a whole blood sample through a separating filter formed of a blood cell separating layer composed mainly of fibers impregnated with a coating agent. A device includes a blood collector having a needle at one end and a blood suction means at the other end, and a separating filter including a blood cell separating layer, the filter being provided in the intermediate zone of the blood collector, whereby a whole blood sample sucked through the needle by depressurizing the blood suction means is passed through the separating filter.

Process for separating serum and plasma

Hydantoin derivatives and salts thereof, intermediates therefor, process for the preparation thereof, and medicines containing the derivative, wherein said derivatives have the formula wherein one of V and W is hydrogen and the other is a halogenomethyl group, 1 H-tetrazol-5-yl radical, -COOR group, in which R is hydrogen atom, an alkyl group, -(CH2CH2O)nCH3 group (n is an integer of 1 to 113) or substituted phenyl, in which R1 and R2 are same or different independently, each is hydrogen atom, an alkyl group, substituted phenyl or -(CH2CH2O)nCH3 group (n has the meaning as referred to) or R1 may form a heterocyclic ring together with R2 and nitogen or oxygen atom, -CH2OR3 group or in which R3 and Ra are same or different independently and each is hydrogen atom or an alkyl group, X is oxygen or sulfur atom, and Y and Z are same or different independently and each is hydrogen atom, a halogen atom, alkyl group, alkoxy group, alkylmercapto group, nitro radical or -NHR5 group, in which R5 is hydrogen atom or an acyl group. The derivatives and salts thereof are useful for the treatment of complications of diabetes.

Masayasu Kurono

Papers

Total synthesis of racemic aflatoxin B1

Biosyntheses of Monascorubrin and Monascoflavin.

Nature of photochemically induced transannular hydrogen abstractions of taxinines

Process for separating serum and plasma

Hydantoin derivatives for treating complications of diabetes