Matjaž Stenovec

TL;DR: It is shown for the first time that numerous PKH26 nanoparticles are formed during ultracentrifugation-based exosome staining, which are almost indistinguishable from PKH 26-labeled exosomes in terms of size, surface area, and fluorescence intensity.

TL;DR: Astrocytes negatively regulate neurogenesis through the Notch pathway, and endocytosis of Notch ligand Jagged1 in astroCytes and Notch signaling from astrocyte to neural stem/progenitor cells depends on the intermediate filament proteins GFAP and vimentin.

TL;DR: The mechanism of release of atrial natriuretic peptide (ANP) from cultured cortical astrocytes by confocal microscopy was investigated, suggesting that ANP is released by regulated Ca2+-dependent exocytosis from astroCytes.

TL;DR: Analysis of the permeation of FM 4-64 dye and HEPES molecules through spontaneously forming fusion pores in lactotroph vesicles expressing synaptopHluorin, a pH-dependent fluorescent fusion marker, indicates an open fusion pore diameter much smaller than the neuropeptides.

TL;DR: Findings show that astrocyte IFs differentially affect the stimulation‐dependent mobility of vesicles, and propose that upregulation of IFs in pathologic states may alter the function ofAstrocytes by deregulating vesicle trafficking.