New flavonoid glycosides from two Astragalus species (Fabaceae) and validation of their antihyperglycaemic activity using molecular modelling and in vitro studies

Today, skin care products and cosmetic preparations containing natural ingredients are widely preferred by consumers. Therefore, many cosmetic brands are encouraged to offer more natural products to the market, such as plant extracts that can be used for their antiaging, antiwrinkle, and depigmentation properties and other cosmetic purposes. In the current study, the volatile constituents of the hexane-soluble fraction of a Stenocarpus sinuatus (family Proteaceae) leaf methanol extract (SSHF) were analyzed using GC/MS analysis. Moreover, the antiaging activity of SSHF was evaluated through in vitro studies of anti-collagenase, anti-elastase, anti-tyrosinase, and anti-hyaluronidase activities. In addition, an in silico docking study was carried out to identify the interaction mechanisms of the major compounds in SSHF with the active sites of the target enzymes. Furthermore, an in silico toxicity study of the identified compounds in SSHF was performed. It was revealed that vitamin E (α-tocopherol) was the major constituent of SSHF, representing 52.59% of the extract, followed by γ-sitosterol (8.65%), neophytadiene (8.19%), β-tocopherol (6.07%), and others. The in vitro studies showed a significant inhibition by SSHF of collagenase, elastase, tyrosinase, and hyaluronidase, with IC50 values of 60.03, 177.5, 67.5, and 38.8 µg/mL, respectively, comparable to those of the positive controls epigallocatechin gallate (ECGC, for collagenase, elastase, hyaluronidase) and kojic acid (for tyrosinase). Additionally, the molecular docking study revealed good acceptable binding scores of the four major compounds, comparable to those of ECGC and kojic acid. Besides, the SSHF identified phytoconstituents showed no predicted potential toxicity nor skin toxicity, as determined in silico. In conclusion, the antiaging potential of SSHF may be attributed to its high content of vitamin E in addition to the synergetic effect of other volatile constituents. Thus, SSHF could be incorporated in pharmaceutical skin care products and cosmetics after further studies.

https://www.mdpi.com/2223-7747/11/7/918/pdf?version=1648567180

GC/MS Profiling, Anti-Collagenase, Anti-Elastase, Anti-Tyrosinase and Anti-Hyaluronidase Activities of a Stenocarpus sinuatus Leaves Extract

HPLC analysis and antioxidant activity of Ulmus davidiana and some flavonoids

Objectives This study was a review of the literature published on the efficacy of Rehmanniae Radix to obtain information to provide both a foundation for its clinical use and directions for future studies. Methods This study selected 30 publications registered to databases or published in Korea by March 13, 2017, by searching electronic journal databases, i.e., NDSL, OASIS, RISS, and KISS, in order to review the efficacy of Rehmanniae Radix. The search was conducted using the keywords '', '', 'Rehmannia Glutinosa', and 'Rehmanniae Radix', and the search results were compiled and analyzed. Results The selected publications included antioxidant effects (12 publications), data involving blood glucose reduction (4 publications), effects on the autonomic nervous system (3 publications), and effects on the human body (11 publications). Conclusion The results of this literature review indicate that Rehmanniae Radix is effective in treating patients with various inflammatory and metabolic diseases, such as high blood pressure and diabetes. Based on these results, we believe that Rehmanniae Radix can be used effectively to treat patients with such diseases.

Rehmanniae Radix, an Effective Treatment for Patients with Various Inflammatory and Metabolic Diseases: Results from a Review of Korean Publications

The nutraceutical role of omija (Schizandra chinensis Baillon) water extract (OWE) was determined through the analysis of antioxidant activity, nitrite scavening activity, and xanthine oxdiase and α-glucosidase inhibitory effects Antioxidant activity of OWE was measured by using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical scavenging activity and superoxide dismutase-like activity (SODA) DPPH radical scavenging activity and SODA increased in a dose-dependent manner, and was about 490% at 25 ㎎/mL and 692% at 5 ㎎/mL, respectively The xanthine oxidase and α-glucosidase inhibitory activities of OWE were about 888% and 862% at 1 ㎎/mL, respectively Nitrite scavenging activity of OWE was about 549%, 424%, and 342% on pH 12, 30, and 60 at 1 mg/mL, respectively These results suggest that OWE has a strong antioxidant activity, and xanthine oxidase and α-glucosidase inhibitory effects

오미자 열수 추출물의 항산화 활성과 아질산 소거능 및 α-Glucosidase 저해 효과

− The antioxidant and anti-diabetes effects were evaluated by the ethanol extracts from Astragalus membranaceus root classified by years using through DPPH radical scavenging activity, hydroxyl radical (·OH) scavenging activity, total phenolic and flavonoid contents, reducing power activity, α-amylase and α-glucosidase inhibitory activities. IC 50 values for DPPH radical scavenging activity of ethanol extract from 6 years old root (749.25 μg/mL) was higher than 1 year (1452.67 μg/mL) and 3 years old root (1095.61 μg/mL). 6 years old root showed better effects in ·OH scavenging activity (IC 50 : 10.58 μg/mL), reducing power, total phenolic contents (26.13±0.79 Tan μg/mg, 24.03±0.74 Cat μg/mg) α-amylase (33.33±0.55%) and α-glucosidase inhibitory activity (49.71±1.01%). On the other hand, total flavonoid compound contents were estimated much higher in 1 year old root (44.93±1.35 Que μg/mg, 70.32±2.03 Rut μg/mg) than others. Based on these results, It was suggested that 6 years old root of A. membranaceus has a potential candidate for functional cosmetic and medicine.

Antioxidant and Antidiabetic Effects of Various Sections of Astragalus membranaceus

To discover the sources with antioxidant and tyrosinase inhibitory activities in Korean traditional medicines, 10 extract of medicinal plants were screened for their potential to scavenge stable 1,1diphenyl-2-picryhydrazyl (DPPH) free radical, inhibit hydroxyl radical (·OH), total phenolic content, and inhibition of tyrosinase. The potency of DPPH radical scavenging activity was shown in the extract of Ulmus davidiana var. japonica Nakai that has a greater effect with IC50 values of 6.49 ± 5.43 μg/mL, than BHA (IC50= 20.99 ± 0.74 μg/mL), L-ascorbic acid (IC50= 20.59 ± 1.06 μg/ mL), and α-tocopherol (IC50= 25.55 ± 0.26 μg/mL) as a positive control. The ·OH scavenging activities were observed in the plants tested. Acanthopanax senticosus, Cirsium setiders, U. davidiana exhibited scavenging activity of more than 60% at 500 μg/mL. The scavenging activity (%) of BHA and a-tocopherol were 64.32 and 55.87% at 500 μg/mL, respectively. The total phenolic content was determined, in order to assess its effect on the extract’s antioxidant activity. The total phenoic content of 33.37 ± 0.52 mg/g was conformed by methanolic extract of U. davidiana. The U. davidiana and Morus bombycis exhibited tyrosinase inhibitory activity with a 34.28 ± 1.32 and 75.57 ± 1.10%, respectively. In particular, M. bombycis has stronger tyrosinase inhibitory activity than albutin with 36.48 ± 3.56% as a positive control. This work showed that the inhibitory abilities of Korean medicinal plants, such as U. davidiana and M. bombycis, on DPPH free radical, inhibit hydroxyl radical (·OH), and inhibition of tyrosinase and total phenolic content, can be useful in the prevention and treatment of free radical-relate disease. Investigations into further isolation of inhibitory principles of U. davidiana and M. bombycis are now in progress.

Antioxidative Activities and Tyrosinase Inhibitory Effects of Korean Medicinal Plants

Aralia elata seeds were successively extracted with water, methanol, ethanol, acetone and chloroform. The crude extracts were investigated for antioxidant and antidiabetic activities. The antioxidant properties of various extracts were evaluated by antioxidant tests, such as DPPH free radical-scavenging activity, hydroxyl radical-scavenging assay, metal-chelating activity, lipid peroxidation inhibition activity and reducing power assay. The 70% methanol extract exhibited the highest activity in the in vitro models of DPPH free radical-scavenging activity, metal-chelating activity, and reducing power assay. Acetone extract showed good effects on lipid peroxidation inhibition and hydroxyl radical-scavenging assay at a low concentration. In addition, the -glucosidase inhibition assay showed that 70% methanol extract had the highest activity. These results indicate the high possibility of using A. elata seeds for medical application due to their efficient antioxidant properties.

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Antioxidant and Antidiabetic Activities of Aralia elata Seeds

From the EtOAc fraction of the MeOH extract of Ulmus davidiana, (-)-catechin (1), (-)-catechin-7-O-β-D-apiofuranoside (2), and (-)-catechin-7-O-β-D-xylopyranoside (3) were isolated and characterized on the basis of ¹H-and ∨13C-NMR, and FABMS spectral data. Compounds 1-3 showed more strong reducing power activities than α-tocopherol, a positive control.

Reducing Power and α-Glucosidase Inhibitory profiles of (-)-Catechin and Its glycoside

국화과 식물인 민들레, 엉겅퀴, 그리고 고려엉겅퀴를 대상으로 항당뇨 활성 측정 방법 중 하나인 흰쥐 수정체 aldose reductase 저해 활성을 측정하였다. 여러 부위별 추출물 중 민들레 지상부 메탄올 추출물이 $IC_{50}\;8.71\;{\mu}g/mL$ 로서 뛰어난 저해 활성을 보였다. 국화과 식물에서 분리된 대표적인 화합물인 silymarin과 민들레에서 분리한 luteolin(1)그리고 고려 엉겅퀴에서 분리된 syringin(2)을 대상으로 aldose reductase저해 활성을 측정한 결과 silymarin과 luteolin은 뛰어난 aldose reductase 저해 활성을 보였다. 【To evaluated active principles for diabetic compolications from the Taraxacum mongolicum (T. mongolicum), Cirsium japonicum (C japonicum), and Cirsium setidens (C. setidens) and its constituents, as well as silymarin tested for their effect on rat lens aldose reductase. As a result, the MeOH extract of T. mongolicum ( $IC_{50}\;8.71\;{\mu}g/mL$ ) have strong lens aldose reductase inhibition abilities. Also, Aldose reductase inhibitory showed an isolated luteolin ( $IC_{50}\;20\;{\mu}M$ ) from the T. mongolicum and sliymarin ( $IC_{50}\;13\;{\mu}M$ ).】

/pdf/rat-lens-aldose-reductase-inhibitory-of-taraxacum-mongolicum-4k4qf8y7vt.pdf

Mee-Jung Jung

Papers

Antioxidant and Antidiabetic Effects of Various Sections of Astragalus membranaceus

Antioxidative Activities and Tyrosinase Inhibitory Effects of Korean Medicinal Plants

Antioxidant and Antidiabetic Activities of Aralia elata Seeds

Reducing Power and α-Glucosidase Inhibitory profiles of (-)-Catechin and Its glycoside

Rat Lens Aldose Reductase inhibitory of Taraxacum mongolicum and two Cirsium species