Showing papers by "Michael Detmar published in 1988"
01 Jan 1988
TL;DR: The recently developed technique or organotypical keratinocyte cultures which results in epidermal differentiation in vitro which is very similar to the in vivo situation was used.
Abstract: Compounds which increase intracellular cAMP levels are used to stimulate proliferation of keratinocytes in culture. For example, in conventional keratinocyte cultures cholera toxin is used for this purpose (Karasek 1983). On the other hand, the effects of cholera toxin are irreversible (Gill 1977). When we replaced in conventional cultures of human keratinocytes cholera toxin by forskolin, a cardiotonic diterpene which activates adenylate cyclase by a reversible manner (Daly 1984), we found growth stimulation up to 500% which was associated with significantly increased intracellular cAMP levels (Artuc et al. 1987). Because we were also interested in the effect of forskolin on cell differentiation we used the recently developed technique or organotypical keratinocyte cultures which results in epidermal differentiation in vitro which is very similar to the in vivo situation (Fusenig et al. 1983).