Showing papers by "Michael Goodfellow published in 1977"
TL;DR: Menaquinones were the only isoprenoid quinones found in 48 corynebacteria and actinomycete strains examined, and Actinomadura dassonvillei strains had a characteristic pattern of di-, tetra- and hexahydromenaquinONES with 10 isoprene units which was slightly different from the pattern in mixtures of similar quInones from Actinomyces israelii and Act inomyces viscosus.
Abstract: Menaquinones were the only isoprenoid quinones found in 48 corynebacteria and actinomycete strains examined. Dihydromenaquinones having nine isoprene units were the main components isolated from Gordona, Mycobacterium, Corynebacterium bovis, Corynebacterium glutamicum and a strain labelled Nocardia farcinica, but dihydromenaquinones having eight isoprene units were characteristic of other Corynebacterium species and representatives of the 'rhodochrous' complex. Tetrahydromenaquinones having six and eight isoprene units were found in Nocardia strains and in a single strain of Micropolyspora brevicatena, which also contained mycolic acids similar in chain length to those of Nocardia. Menaquinones having nine isoprene units with from one to five double bonds hydrogenated were the main components in Actinomadura madurae, Actinomadura pelletieri, Micropolyspora faeni, Oerskovia turbata and Streptomyces strains. Actinomadura dassonvillei strains had a characteristic pattern of di-, tetra- and hexahydromenaquinones with 10 isoprene units which was slightly different from the pattern in mixtures of similar quinones from Actinomyces israelii and Actinomyces viscosus.
2,024 citations
TL;DR: Unidentified glycolipids were detected in the lipids of a majority of the organisms investigated and phosphatidylglycerol was present in substantial amounts in extracts of two strains of Bacterionema matruchotii and in reduced proportions in strains of several other species.
Abstract: Strains representing the mycolic acid-containing taxa Nocardia, Mycobacterium, Gordona, Corynebacterium, Bacterionema, and the “rhodochrous” complex were analysed for polar lipids by two-dimensional, thin-layer chromatography; diphosphatidylglycerol and phosphatidylinositol were found in all strains and phosphatidylethanolamine was absent only in extracts of Corynebacterium and Bacterionema. Mono- and diacyl phosphatidylinositol dimannosides were present in all Nocardia, Mycobacterium, Corynebacterium, and rhodochrous strains and in the single strain of Gordona aurantiaca examined; Bacterionema and strains of the other Gordona species had only a monoacyl phosphatidylinositol dimannoside. Phosphatidylglycerol was present in substantial amounts in extracts of two strains of Bacterionema matruchotii and in reduced proportions in strains of several other species. Unidentified glycolipids were detected in the lipids of a majority of the organisms investigated.
781 citations
TL;DR: The good correlation between the numerical analysis and chemo-taxonomic, serological and genetical data collected from previous studies provides sufficient evidence for raising the "rhodochrous" taxon to generic status.
Abstract: SUMMARY: A numerical taxonomic classification study was carried out on 177 strains representing the ‘rhodochrous’ complex and the genera Gordona, Mycobacterium and Nocardia. The strains were examined for 92 unit characters and the data were analysed by computer. Three clusters were defined at the 75 to 80% similarity level. The first was a heterogeneous cluster corresponding to the ‘rhodochrous’ taxon whereas the other two contained Mycobacterium and Nocardia strains respectively. The good correlation between the numerical analysis and chemo-taxonomic, serological and genetical data collected from previous studies provides sufficient evidence for raising the ‘rhodochrous’ taxon to generic status. We consider the generic name Rhodococcus Zopf to have priority over Proactinomyces (Jensen) Bradley & Bond, Jensenia Bisset & Moore and Gordona Tsukamura. In addition to the type species, Rhodococcus rhodochrous, nine species are recognized: R. bronchialis, R. coprophilus, R. corallinus, R. erythropolis, R. equi, R. rhodnii, R. rubrus, R. rubropertinctus and R. terrae.
291 citations
TL;DR: The genus Bacterionema should be transferred from the family Actinomycetaceae to the Coryneform Group of Bacteria on the basis of lipid data and results of earlier studies.
Abstract: SUMMARY: Whole-organism methanolysates of bacterionemae contained mycolic acids in addition to other long-chain fatty acids. These mycolic acids were similar in general structure and overall size to those found in strains of Corynebacterium diphtheriae and Corynebacterium xerosis. The long-chain fatty acids of bacterionemae, mainly straight-chain saturated and unsaturated acids, were similar to those of certain coryneform bacteria including C. diphtheriae. On the basis of these lipid data, and results of earlier studies, we recommend that the genus Bacterionema be transferred from the family Actinomycetaceae to the Coryneform Group of Bacteria.
41 citations
TL;DR: The degree of binding was determined between deoxyribonucleic acid (DNA) preparations from nocardiae and “rhodochrous” strains and uracil-labeled DNA from three reference strains and there was evidence that N. asteroides is genetically heterogeneous.
Abstract: The degree of binding was determined between deoxyribonucleic acid (DNA) preparations from nocardiae and “rhodochrous” strains and uracil-labeled DNA from three reference strains, Nocardia asteroides N668 and rhodochrous strains N11 and N54. In all cases, good congruence was found between the DNA reassociation data and that from numerical phenetic studies. Only a small degree of nucleotide sequence homology was found between the N. asteroides reference system and the other taxa studied, and there was evidence that N. asteroides is genetically heterogeneous. The moles percent guanine plus cytosine for the rhodochrous strains was within the range 58 to 67; the corresponding range for nocardiae was 64 to 68 mol%.
33 citations
TL;DR: Strains of Listeria denitrificans (E2), Pseudomonas fluorescens (C37 and C92), and Xanthomonas campestris (D119), isolated from the phylloplane of Lolium perenne, were antagonistic to Drechslera dictyoides (DrechSlera) Shoemaker.
Abstract: Strains of Listeria denitrificans (E2), Psendomonas fluorescens (C37 and C92), and Xanthomonas campestris (D119), isolated from the phylloplane of Lolium perenne (S24), were antagonistic to Drechsl...
21 citations
TL;DR: Low proportions of lipids co-chromatographing with, and having the same staining reactions as, phosphatidylethanolamine,osphatidylinositol, and diacyl phosphatids inositol dimannoside were detected in several strains of A. dassonvillei.
Abstract: The polar lipids of Actinomadura dassonvillei, Actinomadura madurae, and Actinomadura pelletieri were analysed by two-dimensional thin-layer chromatography. A. madurae and A. pelletieri had a simple pattern consisting essentially of diphosphatidylglycerol, phosphatidylinositol, and monoacyl phosphatidylinositol dimannoside, but A. dassonvillei strains contained diphosphatidylglycerol, phosphatidylglycerol, a lipid which co-chromatographed with phosphatidylcholine, and a chromatographically mobile unknown phospholipid. Two A. dassonvillei strains had substantial proportions of uncharacterised glycolipids and phosphoglycolipids. Low proportions of lipids co-chromatographing with, and having the same staining reactions as, phosphatidylethanolamine, phosphatidylinositol, and diacyl phosphatidylinositol dimannoside were detected in several strains of A. dassonvillei.
20 citations