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Michał Obuchowski

Researcher at Gdańsk Medical University

Publications -  42
Citations -  940

Michał Obuchowski is an academic researcher from Gdańsk Medical University. The author has contributed to research in topics: Bacillus subtilis & Antigen. The author has an hindex of 15, co-authored 41 publications receiving 782 citations.

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Multicellular behaviour and production of a wide variety of toxic substances support usage of Bacillus subtilis as a powerful biocontrol agent.

TL;DR: Here, the current status of the use of Bacillus subtilis in biocontrol is presented and it is shown that, due to its broad host range, its ability to form endospores and produce different biologically active compounds with a broad spectrum of activity, B. subtillis as well as other Bacilli are potentially useful as biOControl agents.
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Expression and display of UreA of Helicobacter acinonychis on the surface of Bacillus subtilis spores

TL;DR: This heterologous expression system was used to produce the A subunit of the urease of the animal pathogen Helicobater acinonychis and found that UreA was efficiently expressed on the spore coat of B. subtilis when fused to CotB, CotC or CotG.
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CpgA, EF-Tu and the stressosome protein YezB are substrates of the Ser/Thr kinase/phosphatase couple, PrkC/PrpC, in Bacillus subtilis.

TL;DR: It is proposed that the PrpC/PrkC/CpgA system constitutes an important element of a regulatory network involved in the coordination of cell wall expansion and growth in B. subtilis.
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A system of vectors for Bacillus subtilis spore surface display.

TL;DR: A system of vectors designed and constructed for ectopic integration which can be used for spore surface display of heterologous proteins and an example application of presented vector system to display CagA protein of Helicobacter pylori in fusion with CgeA spore coat protein is described.
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Importance of eps genes from Bacillus subtilis in biofilm formation and swarming.

TL;DR: The results obtained showed that mutations inepsJ-epsN, but not in the last gene of theeps operon (epsO), have a severe effect on pellicle formation under all tested conditions, and the inactivation of 5 out of the 6 genes analysed caused total inhibition of swarming in strain 168 (that does not produce surfactin) on LB medium.