Showing papers by "Min Gu published in 1996"
Book•
01 Jan 1996
TL;DR: Three-dimensional Fourier optics of a thin lens confocal bright-field microscopy with a point detector and a finite-sized detector with high-aperture objectives significance of three-dimensional transfer functions.
Abstract: Three-dimensional Fourier optics of a thin lens confocal bright-field microscopy with a point detector confocal bright-field microscopy with a finite-sized detector confocal single-photon fluorescence microscopy confocal two-photon fluorescence microscopy fibre-optical confocal microscopy confocal microscopy under ultrashort pulse illumination confocal microscopy with high-aperture objectives significance of three-dimensional transfer functions.
270 citations
TL;DR: For a given illumination wavelength the resolution for imaging a thick object in the case of three-photon fluorescence imaging can be improved by as much as 40-50% relative to that in two-phorescence microscopic imaging.
Abstract: Resolution in potential three-photon fluorescence scanning microscopy is discussed in terms of the threedimensional optical transfer function. Images of layers and sharp edges are presented for a comparison of the resolution with that in two-photon fluorescence microscopic imaging. For the same fluorescence wavelength the resolution is almost the same in both cases. However, for a given illumination wavelength the resolution for imaging a thick object in the case of three-photon fluorescence imaging can be improved by as much as 40-50% relative to that in two-photon imaging.
73 citations
TL;DR: In this paper, the diffraction patterns by circular and serrated apertures under ultrashort pulsed-laser illumination are investigated in detail, showing that the interference fringes caused by the wavelets originating from the aperture can be further reduced when a serrated aperture is used.
Abstract: Fresnel diffraction patterns by circular and serrated apertures under ultrashort pulsed-laser illumination are investigated in detail. A comparison of the diffraction patterns for pulsed and continuous wave illuminations reveals that an ultrashort pulsed beam of 10 fs can result in a significant effect on the distribution of the intensity. As a result a uniform intensity distribution in the transverse direction of the beam can be achieved as a result of the interference of the different frequency components within the pulsed beam. The interference fringes caused by the wavelets originating from the diffraction aperture can be further reduced when a serrated aperture is used.
66 citations
TL;DR: Various practical effects including those of the annular pupils and the size of the confocal pinhole as well as of the numerical aperture of objectives on the image quality are examined xperimentally.
Abstract: We report on confocal scanning imaging through highly scattering media. Various practical effects including those of the annular pupils and the size of the confocal pinhole as well as of the numerical aperture of objectives on the image quality are examined experimentally. The combination of an annular objective with a finite-sized detector may prove advantageous for improving image quality.
60 citations
TL;DR: In this article, the Fresnel diffraction pattern by a circular opaque disk under ultrashort pulsed-laser illumination is found to be significantly different from that under continuous wave (CW) illumination in the region of large Fresnel numbers.
8 citations
TL;DR: In this article, the axial imaging performance of an imaging system consisting of a single lens is discussed for an incident pulsed laser beam of femtoseconds, and both the point spread function and the transfer function for the lens are examined for both circular and annular pupils.
3 citations
2 citations