Showing papers by "Min Wu published in 2009"
TL;DR: A Gram-negative, aerobic, neutrophilic, coccoid bacterium, strain E4A9T, was isolated from a deep-sea sediment sample collected from the East Pacific polymetallic nodule region and represents a novel species in a new genus in the family Erythrobacteraceae.
Abstract: A Gram-negative, aerobic, neutrophilic, coccoid bacterium, strain E4A9T, was isolated from a deep-sea sediment sample collected from the East Pacific polymetallic nodule region. 16S rRNA gene sequence analysis showed that the isolate was related to the type strain of Altererythrobacter epoxidivorans (96.0% sequence similarity). Lower 16S rRNA gene sequence similarities were observed with other members of the genera Altererythrobacter (94.7%), Erythrobacter (94.0-95.4%), Erythromicrobium (94.8%) and Porphyrobacter (94.6-95.1%) of the family Erythrobacteraceae. Phylogenetic analysis including all described species of the family Erythrobacteraceae and several members of the family Sphingomonadaceae revealed that the isolate formed a distinct phylogenetic lineage with the family Erythrobacteraceae. Chemotaxonomic analysis revealed ubiquinone-10 as the predominant respiratory quinone, anteiso-C15:0, iso-C14:0 and iso-C15:0 as major fatty acids, and phosphatidylglycerol as the major polar lipid. The DNA G+C content was 71.5 mol%. The isolate contained carotenoids, but no bacteriochlorophyll a. On the basis of phenotypic and genotypic data presented in this study, strain E4A9T represents a novel species in a new genus in the family Erythrobacteraceae for which the name Croceicoccus marinus gen. nov., sp. nov. is proposed; the type strain is E4A9T (=CGMCC 1.6776T=JCM 14846T).
47 citations
TL;DR: Compared with other gas hydrate-rich ecosystems and different areas of the South China Sea, a distinct microbial community was revealed in this study.
Abstract: Microbial diversity of sediments from the northern slope of the South China Sea was studied by constructing bacterial and archaeal 16S rRNA gene clone libraries. Fourteen bacterial phylogenetic groups were detected, including Gammaproteobacteria, Deltaproteobacteria, Planctomycetes, Alphaproteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Chloroflexi, Acidobacteria, Betaproteobacteria, Nitrospirae, candidate divisions OP8 and OP11, and an unknown group. Gammaproteobacteria was the predominant group in bacterial libraries with the percentage ranging from 31.8% to 63.2%. However, archaeal libraries had relatively lower diversity, with most clones belonging to marine archaeal group I uncultured Crenarchaeota. In addition, two novel euryarchaeal clones were detected not to match any culture-dependent or -independent isolates. Compared with other gas hydrate-rich ecosystems and different areas of the South China Sea, a distinct microbial community was revealed in this study.
32 citations
TL;DR: Two Gram-negative, aerobic, motile, rod-shaped bacteria, designated strains 908033(T) and 908087(T), were isolated from a seawater sample collected from the East China Sea and demonstrated that the new isolates represent two novel species of the genus Pseudidiomarina.
Abstract: Two Gram-negative, aerobic, motile, rod-shaped bacteria, designated strains 908033(T) and 908087(T), were isolated from a seawater sample collected from the East China Sea. Chemotaxonomic characteristics of the two isolates included the presence of iso-C(15 : 0), iso-C(17 : 0) and iso-C(17 : 1)omega9c as the major cellular fatty acids and Q-8 as the predominant ubiquinone. The genomic DNA G+C contents of strains 908033(T) and 908087(T) were 45.5 and 45.2 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the new isolates were related to members of the genus Pseudidiomarina, showing levels of similarity of 95.8-96.6 % with the type strains of recognized species of the genus. The results of DNA-DNA hybridization experiments among these two isolates and Pseudidiomarina sediminum CICC 10319(T), in combination with chemotaxonomic and phenotypic data, demonstrated that the new isolates represent two novel species of the genus Pseudidiomarina, for which the names Pseudidiomarina donghaiensis sp. nov. (type strain 908033(T)=CGMCC 1.7284(T)=JCM 15533(T)) and Pseudidiomarina maritima sp. nov. (type strain 908087(T)=CGMCC 1.7285(T)=JCM 15534(T)) are proposed.
24 citations
TL;DR: A Gram-negative, aerobic, neutrophilic, rod-shaped bacterium, designated strain CN85(T), was isolated from a sediment sample collected from the East China Sea and was subjected to a polyphasic taxonomic characterization and phenotypic and genotypic data showed that Q-8 was the predominant respiratory quinone and that iso-C,iso-C(11 : 0) and C were the major fatty acids.
Abstract: A Gram-negative, aerobic, neutrophilic, rod-shaped bacterium, designated strain CN85(T), was isolated from a sediment sample collected from the East China Sea and was subjected to a polyphasic taxonomic characterization. This isolate grew in the presence of 0.5-6.0 % (w/v) NaCl and at 20-40 degrees C; optimum growth was observed with 3 % (w/v) NaCl and at 35 degrees C. Chemotaxonomic analysis showed that Q-8 was the predominant respiratory quinone and that iso-C(15 : 0), iso-C(11 : 0) 3-OH, iso-C(17 : 1)omega9c, iso-C(17 : 0), iso-C(11 : 0) and C(16 : 0) were the major fatty acids. The G+C content of the genomic DNA was 57.8 mol%. On the basis of 16S rRNA gene sequence analysis, the isolate was affiliated to the genus Microbulbifer. Strain CN85(T) exhibited most phylogenetic affinity with respect to the type strain of Microbulbifer maritimus (97.0 % sequence similarity) and showed less than 97 % sequence similarity with respect to other described Microbulbifer species with known 16S rRNA gene sequences. The DNA-DNA hybridization between strain CN85(T) and M. maritimus JCM 12187(T) was 44 %. On the basis of phenotypic and genotypic data, strain CN85(T) represents a novel species of the genus Microbulbifer, for which the name Microbulbifer donghaiensis sp. nov. is proposed. The type strain is CN85(T) (=CGMCC 1.7063(T) =JCM 15145(T)).
21 citations
TL;DR: Two strains, CN44(T) and CN47(T), isolated from marine sediment of the East China Sea, were characterized by using a polyphasic approach and represent two novel species within the genus Marinobacterium, for which the names Marinobacteria nitratireducens sp.
Abstract: Two strains, CN44(T) and CN47(T), isolated from marine sediment of the East China Sea, were characterized by using a polyphasic approach. The isolates were Gram-negative, strictly aerobic, non-spore-forming rods. The chemotaxonomic characteristics of these isolates included the presence of C(18 : 1)omega7c, C(16 : 0), iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c and C(10 : 0) 3-OH as the major cellular fatty acids and Q-8 as the predominant ubiquinone. The DNA G+C contents of strains CN44(T) and CN47(T) were 62.5 and 56.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain CN44(T) was related to members of the genus Marinobacterium. The most closely related described organism was the type strain of Marinobacterium rhizophilum (95.3 % sequence similarity). Strain CN47(T) showed the highest sequence similarity to the type strain of Marinobacterium stanieri (97.8 %) and <97 % similarity to other type strains of described Marinobacterium species. The level of DNA-DNA relatedness between strain CN47(T) and M. stanieri DSM 7027(T) was 46 %. On the basis of phenotypic and genotypic properties, strains CN44(T) and CN47(T) represent two novel species within the genus Marinobacterium, for which the names Marinobacterium nitratireducens sp. nov. (type strain, CN44(T) =CGMCC 1.7286(T) =JCM 15523(T)) and Marinobacterium sediminicola sp. nov. (type strain, CN47(T) =CGMCC 1.7287(T) =JCM 15524(T)) are proposed.
19 citations
TL;DR: The isolate was catalase- and oxidase-positive and cells were motile by means of polar flagella, and formed a monophyletic clade adjacent to the type strain of V. agarivorans.
Abstract: Strain CN83T, a Gram-negative, aerobic, rod-shaped bacterium, was isolated from sediment of the East China Sea. The isolate was catalase- and oxidase-positive and cells were motile by means of polar flagella. The DNA G+C content was 44.9 mol%. The major fatty acids were C16:1omega7c and/or iso-C15:0 2-OH, C16:0, C18:1omega7c, C14:0 and C12:0. 16S rRNA gene sequence analysis showed that strain CN83T belonged to the genus Vibrio and had the highest sequence similarity to Vibrio agarivorans (98.4%) and Vibrio campbellii (97.8%). Phylogenetic analysis revealed that strain CN83T formed a monophyletic clade adjacent to the type strain of V. agarivorans. The DNA-DNA hybridization values of strain CN83T with V. agarivorans DSM 13756T and V. campbellii DSM 19270T were 44.6 and 25.5%, respectively. On the basis of the phenotypic and genotypic data, strain CN83T represents a novel species of the genus Vibrio, for which the name Vibrio hangzhouensis sp. nov. is proposed. The type strain is CN83T (=CGMCC 1.7062T=JCM 15146T).
11 citations
TL;DR: The complete sequence of the bop gene, including the 5' and 3' flanking regions of the conserved fragment, was obtained by the ligation-mediated PCR amplification (LPA) approach, providing further insight into the distribution of bop-like genes in the family Halobacteriaceae.
4 citations