Showing papers by "Min Wu published in 2013"

Aequorivita viscosa sp. nov., isolated from an intertidal zone, and emended descriptions of Aequorivita antarctica and Aequorivita capsosiphonis.

Abstract: An aerobic, Gram-stain-negative, short rod-shaped, non-motile and non-sporulating bacterium, designed strain 8-1bT, was isolated from seaweed collected from the intertidal zone of Zhoushan sea area, East China Sea. Strain 8-1bT grew at 4–39 °C (optimum, 28–32 °C) and at pH 6.0–9.5 (optimum, 7.0–8.5), and with 0.5–8 % (w/v) NaCl (optimum, 1–3 %) and 0.5–10 % (w/v) sea salts (optimum, 2–3 %). Analysis of 16S rRNA gene sequences revealed that strain 8-1bT was related closely to Aequorivita capsosiphonis JCM 15070T (96.7 % similarity). The DNA G+C content of strain 8-1bT was 36.6 mol%. Compared with reference strains, cells of strain 8-1bT showed positive activities for H2S production and utilization of d-mannose, dl-lactic acid, l-asparagine and glycyl l-aspartic acid. The major fatty acids of strain 8-1bT were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C17 : 1ω9c. The main respiratory quinone was menaquinone 6. The polar lipids of strain 8-1bT consisted of phosphatidylethanolamine (PE), three uncharacterized aminolipids (AL1–3), four uncharacterized glycolipids (GL1–4) and five uncharacterized lipids (L1–5). Based on the phenotypic and genotypic characterization, strain 8-1bT represents a novel species of the genus Aequorivita , for which the name Aequorivita viscosa sp. nov. is proposed. The type strain is strain 8-1bT ( = CGMCC 1.11023T = JCM 18497T). Emended descriptions of Aequorivita antarctica and Aequorivita capsosiphonis are also presented.

Extensimonas vulgaris gen. nov., sp. nov., a member of the family Comamonadaceae.

Abstract: A novel strain, named S4(T), was obtained from industrial wastewater in Xiaoshan, Zhejiang Province, China Cells were Gram-negative, neutrophilic and non-spore-forming and moved by means of a polar flagellum Normal cells were 08-09 × 13-19 µm and the cells elongated to 10-25 µm when cultivated at high temperatures Strain S4(T) grew at 15-50 °C (optimum at 48 °C), pH 55-85 (optimum 70-75) and 0-2% (optimum 05%) (w/v) NaCl Ubiquinone-8 was the predominant respiratory quinone C16:0, summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH) and C17:0 cyclo were the major cellular fatty acids The major 3-OH fatty acid was C10:0 3-OH The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown aminoglycolipid The genomic DNA G+C content was 688 mol% Based on 16S rRNA gene sequences alignment, the most closely related strains were members of the genera Comamonas (946-956% similarities), Giesbergeria (949-956%), Acidovorax (948-954%), Brachymonas (941-952%) and Macromonas (951%) Phylogenetic analysis showed the closest relatives of strain S4(T) were members of the genus Macromonas Based on phenotypic and phylogenetic characteristics, we suggest that strain S4(T) represents a novel species of a new genus of the family Comamonadaceae, for which the name Extensimonas vulgaris gen nov, sp nov is proposed The type strain of Extensimonas vulgaris is S4(T) (=CGMCC 110977(T)=JCM 17803(T))

Roseivivax pacificus sp. nov., isolated from deep-sea sediment.

Abstract: A Gram-stain-negative, short-rod-shaped bacterium, designated 22DY03T, was isolated from a sediment sample collected from the East Pacific Rise. The isolate required NaCl and grew best with 3–7 % (w/v) sea salts at temperature of between 30 and 35 °C at pH 7.0. It formed non-pigmented colonies and produced exopolysaccharide, but did not produce bacteriochlorophyll a. Strain 22DY03T was positive for hydrolysis of aesculin and Tween 20 and negative for hydrolysis of casein, DNA, gelatin, starch and Tween 40, 60 and 80. The major respiratory quinone was ubiquinone-10. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol, two unidentified phospholipids and four unidentified polar lipids. The major fatty acids were C19 : 0 cyclo ω8c, C18 : 1ω7c and 11-methyl C18 : 1ω7c. The genomic DNA G+C content was 64.6 mol%. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain 22DY03T should be assigned to the genus Roseivivax . The 16S rRNA gene sequence similarities between the isolate and the type strains of species of the genus Roseivivax were in the range of 94.1–95.8 %. On the basis of phenotypic and genotypic data, it is concluded that strain 22DY03T represents a novel species of the genus Roseivivax , for which the name Roseivivax pacificus sp. nov. (type strain 22DY03T = CGMCC 1.12410T = JCM 18866T) is proposed.

Salimesophilobacter vulgaris gen. nov., sp. nov., an anaerobic bacterium isolated from paper-mill wastewater.

Abstract: A novel anaerobic, heterotrophic bacterium, designated strain Zn2T, was isolated from the wastewater of a paper mill in Zhejiang, China. Cells were Gram-type-positive rods, 0.5–0.8 µm wide and 2–4 µm long, and were motile by a lateral flagellum. The ranges of temperature and pH for growth were 10–50 °C and pH 6.0–9.5. Optimal growth occurred at 35 °C and pH 7.3–7.5. The strain did not require NaCl for growth, but its inclusion in the medium improved growth (optimum concentration 6 %). Substrates utilized as sole carbon sources were peptone, tryptone, Casamino acids, d-xylose, salicin, glycerol, formate, acetate and propionate. The main products of carbohydrate fermentation were acetate, formate, propionate and lactate. Elemental sulfur, thiosulfate and Fe(III) were used as electron acceptors, but sulfate, sulfite, nitrate, nitrite and Mn(IV) were not. Growth was inhibited by the addition of 10 µg ampicillin, penicillin, tetracycline or chloramphenicol ml−1. iso-C15 : 0, C14 : 0, C16 : 0, C16 : 1 cis9 and C18 : 1 cis9 were the major fatty acids. Strain Zn2T did not contain any detectable menaquinones or ubiquinones. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, two unknown phospholipids and four unknown glycolipids. The genomic DNA G+C content was 37 mol%, as determined by HPLC. 16S rRNA gene sequence analysis revealed that strain Zn2T was a member of family Clostridiaceae , and was most closely related to the type strains of Geosporobacter subterraneus , Thermotalea metallivorans and Caminicella sporogenes , showing 91.2, 90.3 and 91.1 % sequence similarity, respectively. On the basis of its phenotypic and genotypic properties, strain Zn2T is suggested to represent a novel species of a new genus, for which the name Salimesophilobacter vulgaris gen. nov., sp. nov. is proposed. The type strain of Salimesophilobacter vulgaris is Zn2T ( = DSM 24770T = JCM 17796T).

Oceanirhabdus sediminicola gen. nov., sp. nov., an anaerobic bacterium isolated from sea sediment.

Abstract: A novel anaerobic bacterium, designated NH-JN4T was isolated from a sediment sample collected in the South China Sea. Cells were Gram-stain-positive, spore-forming, peritrichous and rod-shaped (0.5–1.2×2.2–7 µm). The temperature and pH ranges for growth were 22–42 °C and pH 6.0–8.5. Optimal growth occurred at 34–38 °C and pH 6.5–7.0. The NaCl concentration range for growth was 0.5–6 % (w/v) with an optimum of 2.5 %. Catalase and oxidase were not produced. Substrates which could be utilized were peptone, tryptone, yeast extract, beef extract and glycine. Main fermentation products from PYG medium were formate, acetate, butyrate and ethanol. Strain NH-JN4T could utilize sodium sulfite as an electron acceptor. No respiratory quinone was detected. The predominant fatty acids were anteiso-C15 : 0, C16 : 0, iso-C15 : 0, anteiso-C17 : 0 and C16 : 0 DMA. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and glycolipids. The DNA G+C content was 35.8 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain NH-JN4T was a member of family Clostridiaceae , and was most closely related to Clostridium limosum ATCC 25620T, Clostridium proteolyticum DSM 3090T, Clostridium histolyticum ATCC 19401T and Clostridium tepidiprofundi SG 508T, showing 94.0, 93.0, 92.9 and 92.3 % sequence similarity, respectively. On the basis of phenotypic, genotypic and chemotaxonomic properties, strain NH-JN4T represents a novel species of a new genus in the family Clostridiaceae , for which the name Oceanirhabdus sediminicola gen. nov., sp. nov. is proposed. The type strain of the type species is NH-JN4T ( = JCM 18501T = CCTCC AB 2013103T = KCTC 15322T).

Draft Genome Sequence of Amphibacillus jilinensis Y1(T), a Facultatively Anaerobic, Alkaliphilic and Halotolerant Bacterium.

Abstract: The genus Amphibacillus was established in 1990, and seven additional species were described in the past two decades Amphibacillus jilinensis Y1T is a facultatively anaerobic and alkaliphilic bacterium isolated from a soda lake in China Here we describe the structural and genetic features of the draft genome about the type strain Y1T (3,831,075 bp, with a G+C content of 3727%) This is the first genome report of the Amphibacillus genus