/pdf/beck-depression-inventory-iinituitenoyi-kao-cha-3143h0pleh.pdf

Beck Depression Inventory-IIについての一考察

Adipose-derived stem cells (ADSCs) have raised big interest in therapeutic applications in regenerative medicine and appear to fulfill the criteria for a successful cell therapy. Their low immunogenicity and their ability to self-renew, to differentiate into different tissue-specific progenitors, to migrate into damaged sites, and to act through autocrine and paracrine pathways have been altogether testified as the main mechanisms whereby cell repair and regeneration occur. The absence of standardization protocols in cell management within laboratories or facilities added to the new technologies improved at patient's bedside and the discrepancies in cell outcomes and engraftment increase the limitations on their widespread use by balancing their real benefit versus the patient safety and security. Also, comparisons across pooled patients are particularly difficult in the fact that multiple medical devices are used and there is absence of harmonized assessment assays despite meeting regulations agencies and efficient GMP protocols. Moreover, the emergence of the COVID-19 breakdown added to the complexity of implementing standardization. Cell- and tissue-based therapies are completely dependent on the biological manifestations and parameters associated to and induced by this virus where the scope is still unknown. The initial flow chart identified for stem cell therapies should be reformulated and updated to overcome patient infection and avoid significant variability, thus enabling more patient safety and therapeutic efficiency. The aim of this work is to highlight the major guidelines and differences in ADSC processing meeting the current good manufacturing practices (cGMP) and the cellular therapy-related policies. Specific insights on standardization of ADSCs proceeding at different check points are also presented as a setup for the cord blood and bone marrow.

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Overview of current adipose-derived stem cell (ADSCs) processing involved in therapeutic advancements: flow chart and regulation updates before and after COVID-19.

Adult mesenchymal stem cells and their exosomes: Sources, characteristics, and application in regenerative medicine.

In late 2019, a novel coronavirus (SARS-CoV-2) emerged in Wuhan, capital city of Hubei province in China. Cases of SARS-CoV-2 infection quickly grew by several thousand per day. Less than 100 days later, the World Health Organization declared that the rapidly spreading viral outbreak had become a global pandemic. Coronavirus disease 2019 (COVID-19) is typically associated with fever and respiratory symptoms. It often progresses to severe respiratory distress and multi-organ failure which carry a high mortality rate. Older patients or those with medical comorbidities are at greater risk for severe disease. Inflammation, pulmonary edema and an over-reactive immune response can lead to hypoxia, respiratory distress and lung damage. Mesenchymal stromal/stem cells (MSCs) possess potent and broad-ranging immunomodulatory activities. Multiple in vivo studies in animal models and ex vivo human lung models have demonstrated the MSC’s impressive capacity to inhibit lung damage, reduce inflammation, dampen immune responses and aid with alveolar fluid clearance. Additionally, MSCs produce molecules that are antimicrobial and reduce pain. Upon administration by the intravenous route, the cells travel directly to the lungs where the majority are sequestered, a great benefit for the treatment of pulmonary disease. The in vivo safety of local and intravenous administration of MSCs has been demonstrated in multiple human clinical trials, including studies of acute respiratory distress syndrome (ARDS). Recently, the application of MSCs in the context of ongoing COVID-19 disease and other viral respiratory illnesses has demonstrated reduced patient mortality and, in some cases, improved long-term pulmonary function. Adipose-derived stem cells (ASC), an abundant type of MSC, are proposed as a therapeutic option for the treatment of COVID-19 in order to reduce morbidity and mortality. Additionally, when proven to be safe and effective, ASC treatments may reduce the demand on critical hospital resources. The ongoing COVID-19 outbreak has resulted in significant healthcare and socioeconomic burdens across the globe. There is a desperate need for safe and effective treatments. Cellular based therapies hold great promise for the treatment of COVID-19. This literature summary reviews the scientific rationale and need for clinical studies of adipose-derived stem cells and other types of mesenchymal stem cells in the treatment of patients who suffer with COVID-19.

/pdf/rationale-for-the-clinical-use-of-adipose-derived-4spxfymnob.pdf

Rationale for the clinical use of adipose-derived mesenchymal stem cells for COVID-19 patients.

Bone marrow mesenchymal stem/stromal cells (BMSCs), which are known as multipotent cells, are widely used in the treatment of various diseases via their self-renewable, differentiation, and immunomodulatory properties. In-vitro and in-vivo studies have supported the understanding mechanisms, safety, and efficacy of BMSCs therapy in clinical applications. The number of clinical trials in phase I/II is accelerating; however, they are limited in the size of subjects, regulations, and standards for the preparation and transportation and administration of BMSCs, leading to inconsistency in the input and outcome of the therapy. Based on the International Society for Cellular Therapy guidelines, the characterization, isolation, cultivation, differentiation, and applications can be optimized and standardized, which are compliant with good manufacturing practice requirements to produce clinical-grade preparation of BMSCs. This review highlights and updates on the progress of production, as well as provides further challenges in the studies of BMSCs, for the approval of BMSCs widely in clinical application.

https://www.mdpi.com/1422-0067/21/3/708/pdf

An Update on the Progress of Isolation, Culture, Storage, and Clinical Application of Human Bone Marrow Mesenchymal Stem/Stromal Cells.

Adipose tissue is now on the top one of stem cell sources regarding its accessibility, abundance, and less painful collection procedure when compared to other sources. The adipose derived stem cells (ADSCs) that it contains can be maintained and expanded in culture for long periods of time without losing their differentiation capacity, leading to large cell quantities being increasingly used in cell therapy purposes. Many reports showed that ADSCs-based cell therapy products demonstrated optimal efficacy and efficiency in some clinical indications for both autologous and allogeneic purposes, hence becoming considered as potential tools for replacing, repairing, and regenerating dead or damaged cells. In this review, we analyzed the therapeutic advancement of ADSCs in comparison to bone marrow (BM) and umbilical cord (UC)-mesenchymal stem cells (MSCs) and designed the specific requirements to their best clinical practices and safety. Our analysis was focused on the ADSCs, rather than the whole stromal vascular fraction (SVF) cell populations, to facilitate characterization that is related to their source of origins. Clinical outcomes improvement suggested that these cells hold great promise in stem cell-based therapies in neurodegenerative, cardiovascular, and auto-immunes diseases.

https://www.mdpi.com/1422-0067/20/10/2523/pdf

Regenerative Capacity of Adipose Derived Stem Cells (ADSCs), Comparison with Mesenchymal Stem Cells (MSCs).

Objective The present study compared the ability of two contemporary theories of suicidal behavior-the interpersonal and escape theories of suicide-to predict suicidal ideation. The interpersonal theory proposes that the interaction of perceived burdensomeness and thwarted belongingness predicts suicidal ideation. The escape theory proposes that feelings of failure predict suicidal ideation and that escape motivation mediates this relationship. The present study intended to determine which of the two theories more successfully explains suicidal ideation. Method A sample of 306 students from elite schools in Morocco (193 women, Mage = 21.21 years, predominantly Muslims) completed a questionnaire assessing feelings of failure and escape motivation, perceived burdensomeness and thwarted belongingness, suicide ideation, and control variables. Results In line with previous research, separate tests revealed support for the two theories. Interestingly, when entered simultaneously in a multiple regression analysis, the two frameworks explained a unique and cumulative part of the variance in suicidal ideation. Moreover, the effects remained significant after controlling for past suicide attempts, depression, hopelessness, and stress. Conclusions The findings suggest that combining the interpersonal and escape theories of suicide could help better explain the emergence of suicidal ideation among college students.

/pdf/suicidal-ideation-in-elite-schools-a-test-of-the-4nej7uy9kk.pdf

Suicidal Ideation in Elite Schools: A Test of the Interpersonal Theory and the Escape Theory of Suicide.

Pandémie COVID-19 et responsabilité sociale des étudiants en médecine en formation : quelles leçons pour l’avenir ?(Cas de la Faculté de médecine de Marrakech)

The data collecting process was carried out using the French version of the STEPwise approach developed by the WHO to monitor NonCommunicable Diseases risk factors, producing thus standardized data and allowing wide comparability across similar studies. The investigation was conducted through on-site and online configurations. We only relied on the first and second sequences of the STEPS questionnaire in order to collect behavioral and physical data, on which our analysis was based. The choice of the population of Moroccan high intellectual potential youth is interesting, as they represent future physicians and leading engineers of tomorrow.

/pdf/cardiovascular-diseases-risk-factors-among-young-adults-a-13y526zyau.pdf

Cardiovascular Diseases Risk Factors Among Young Adults: A STEPwise-Approach-Based Study in Three University Setups in Morocco

A novel virus has emerged to the world causing a new pandemic of respiratory infection. On February 2020, the World Health Organization named it Corona disease 2019. The emergence of this epidemic was first reported in Wuhan, China in late December 2019, before it spread to other countries. In response to this pandemic, the World Health Organization has stressed the need for strengthening health professionals worldwide. Indeed, adequate protective measures can help protect patients and staff from the transmission of a highly infectious disease.
Managing COVID-19 patients exposes healthcare professionals to the risk of contracting the disease and transmitting it to those around them. This risk is very high because of the mode of transmission of SARS-CoV-2, which is transmitted by inhalation, contact with droplets and infected surfaces which makes it a very contagious disease. Thus, adequate precautions in healthcare establishments can help mitigate this risk, such as adopting several protective measures including personal protective equipment. By the same vein, healthcare professionals run a high risk of anxiety, stress, depression and burnout when managing COVID-19 patients. Therefore, the protection of the mental health of these health workers also includes psychological support measures.

In this regard, this article purports to describe the health and safety measures related to the prevention of the risk of COVID-19 infection to health professionals in China and Morocco. On the other hand, it seeks to present the psychological support measures for these agents in the two aforementioned countries.

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Mohamed Amine

Papers

Regenerative Capacity of Adipose Derived Stem Cells (ADSCs), Comparison with Mesenchymal Stem Cells (MSCs).

Suicidal Ideation in Elite Schools: A Test of the Interpersonal Theory and the Escape Theory of Suicide.

Pandémie COVID-19 et responsabilité sociale des étudiants en médecine en formation : quelles leçons pour l’avenir ?(Cas de la Faculté de médecine de Marrakech)

Cardiovascular Diseases Risk Factors Among Young Adults: A STEPwise-Approach-Based Study in Three University Setups in Morocco

Protective Measures of Healthcare Professionals to Face COVID-19: Case of China and Morocco