Industrial applications of microbial lipases

The text consists of two sections, one for those studying or practicing diagnostic radiology, nuclear medicine and radiation oncology; the other for those engaged in the study or clinical practice of radiation oncology--a new chapter, on radiologic terrorism, is specifically for those in the radiation sciences who would manage exposed individuals in the event of a terrorist event. The 17 chapters in Section I represent a general introduction to radiation biology and a complete, self-contained course especially for residents in diagnostic radiology and nuclear medicine that follows the Syllabus in Radiation Biology of the RSNA. The 11 chapters in Section II address more in-depth topics in radiation oncology, such as cancer biology, retreatment after radiotherapy, chemotherapeutic agents and hyperthermia.

Radiobiology for the Radiologist

Organic solvent-tolerant enzymes

A quantitative starch-iodine method for measuring alpha-amylase and glucoamylase activities.

Abstract The use of organic solvents as reaction media for enzymatic reactions provides numerous industrially attractive advantages compared to traditional aqueous reaction systems. Despite the advantages, native enzymes almost universally exhibit low activities and/or stabilities in the presence of organic solvents. This inactivation of the enzymes by organic solvents results in significant limitation of the enzymatic reaction process. Numerous attempts have been made to improve enzyme activity and stability in the presence of organic solvents using methods based on protein engineering and chemical or physical modification. Most enzymes used in these studies did not originally exhibit high activity and stability in the presence of organic solvents because they were not screened as organic solvent-tolerant enzymes. Recently, various attempts have been made to screen enzymes that naturally possess organic solvent-tolerance form various microorganisms including organic solvent-tolerant bacteria, thermophiles, halophiles and mesophiles. These organic solvent-tolerant enzymes are expected to have potential for applications in industrial chemical processes.

[Organic solvent-tolerant enzymes].

RESEARCH ARTICLE - Potential application of protease isolated from Pseudomonas aeruginosa PD100

Potential application of protease isolated from Pseudomonas aeruginosa PD100

Purification and characterization of an extracellular α-amylase from Bacillus subtilis AX20

The water extracts of propolis (WEP) could inhibit growth of different cell lines namely McCoy, HeLa, SP2/0, HEp-2, and BHK21 and stimulate growth of normal cell named human lymphocyte, rat kidney, rat liver, and rat spleen. In these experiments 1 and 2 mg of WEP were added to 1 ml RPMI media with 5% FCS. Cell counts and cell viability of propolis-treated and propolis-free cells were assessed by Trypan blue dye exclusion test and MTT assay. The results showed that in case of McCoy, HeLa, SP20, HEp-2, and BHK21 cell lines, the water extracts of propolis could inhibit cell growth as well as reduction on size of the cells. In contrast the same amount of WEP could stimulate growth of normal cells up to 60% with the same concentration used for cell lines. Thus our study indicates that although WEP consists only of the soluble part of propolis, it enables to inhibit different cell lines and increase growth of normal cells. This indicates also that WEP contains the specific compounds with bioactivity against cell lines. Although propolis contain different number of compounds it is clear that WEP has enough biological compounds useful for the treatment of some diseases, medical and related applications.

/pdf/effect-of-the-water-extracts-of-propolis-on-stimulation-and-24pcw2jm3h.pdf

Effect of the water extracts of propolis on stimulation and inhibition of different cells

In the present study, water extract of dried fruit of Zyzyphus Jujube was tested for its possible anticancer effect and induction of apoptosis on human tumor cell lines, HEp-2, HeLa and Jurkat cell lines. The inhibitory effect of water extract of this fruit on cell proliferation was assessed by MTT colorimetric assay. The induction of apoptosis of this extract was analyzed by DNA fragmentation analysis. Zyzyphus Jujube extract showed inhibitory effects on mentioned cell lines. Jurkat leukemic line was found the most sensitive cells with IC50 of 0.1 μg mL−1. Our study also showed a typical DNA laddering in this cell line. The present study showed cytotoxic activity of Zyzyphus Jujube on tumor cells. Although Zyzyphus Jujube has useful compounds for medical applications.

/pdf/evaluation-of-inhibitory-effect-and-apoptosis-induction-of-9bwx6dw3o0.pdf

Mohsen Fathi Najafi

Papers

RESEARCH ARTICLE - Potential application of protease isolated from Pseudomonas aeruginosa PD100

Potential application of protease isolated from Pseudomonas aeruginosa PD100

Purification and characterization of an extracellular α-amylase from Bacillus subtilis AX20

Effect of the water extracts of propolis on stimulation and inhibition of different cells

Evaluation of inhibitory effect and apoptosis induction of Zyzyphus Jujube on tumor cell lines, an in vitro preliminary study