. Fadhli N, Farid M, Rafiuddin, Efendi R, Azrai M, Anshori MF. 2020. Multivariate analysis to determine secondary characters in selecting adaptive hybrid corn lines under drought stress. Biodiversitas 21: 3617-3624. The development of adaptive hybrid corn varieties under drought stress needs an effective selection. Multivariate analysis has been reported can increase the effectiveness of selection in plants by attaching the secondary characters in the selection. Therefore, this concept also can be applied to develop adaptive corn varieties under drought stress. The objectives of this study are to determine the main secondary characters and select the best hybrid lines adaptive to drought stress. The experiment was arranged by a nested design, where replications nested under two environmental conditions, namely normal and drought stress. The main factor was genotypes consisted of 30 genotypes and was repeated three times. Moreover, the observations of this research consisted of 20 variables. The result of this research showed that the weight of harvested cob was an effective secondary character as a selection criterion along with productivity in selecting adaptive maize genotypes under drought stress. The number of green leaves was the character outside of the yield component could be as an alternative secondary character besides the weight of harvested cob. The selection results based on the weight of the harvested cob and productivity resulted in 12 hybrid corn lines considered adaptive to drought stress.

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Multivariate analysis to determine secondary characters in selecting adaptive hybrid corn lines under drought stress

Garden pea is an important off-season vegetable in the hills of North Western Himalayas that provides high remuneration to growers. Lack of high yielding wider adaptive varieties restricts its productivity. To identify high yielding stable genotypes across environments, 48 lines were evaluated in Alpha Lattice design over seven diverse environments spreading in five locations from sub-tropical to dry temperate conditions of Himachal Pradesh during winter 2016–2017 (5 environments) and one location each during summer 2017 and winter 2017–2018. The highest mean pod yield over environments was produced by SP-3 (83.35 g) which was 20% higher over check Pb-89 while SP-6 (79.45 g) and SP-22 (76.90 g) got second and third rank with significant increase of 15 and 11% for pod yield over the check, respectively. The joint regression analysis depicted significant G × E (linear) interaction for pods/plant and pod yield/plant. Of the seven genotypes depicting stability for pod yield, SP-6 showed stability (bi = 1 and S2di = 0) for all the traits whereas SP-22 showed the same for seeds/pod and shelling percentage. G + GE biplot described Dhaulakuan as the most representative and discriminating environment for pod yield. In the mean vs. stability GGE biplot, SP-6, SP-22 and SP-17 were highly stable while SP-3 possessed medium stability. ‘Which won where’ polygon view represented SP-3, SP-6 and SP-22 as most stable for yield and pod attributes across environments, constellated into majority of the environments and can be released for commercial cultivation.

Stability analysis of garden pea (Pisum sativum L.) genotypes under North Western Himalayas using joint regression analysis and GGE biplots

. Purba JH, Wahyuni PS, Zulkarnaen, Sasmita N, Yuniti IGD, Pandawani NP. 2020. Growth and yield response of shallot (Allium ascalonicum L. var. Tuktuk) from different source materials applied with liquid biofertilizers. Nusantara Bioscience 12: 127-133. This research was to examine growth and yield of shallots using different sources of propagation material, namely true shallot seed (TSS) and bulbs. Soil biological fertility, which was generally low, was improved by the addition of liquid organic fertilizer. The purpose of this study a) to determine the differences in the propagation of plants from seeds and bulbs of shallot Tuktuk varieties, and b) to determine the effect of liquid biofertilizer maxigrow and rhizobacteria. The study used a one-factor randomized design. The results showed that the growth and yield of shallots propagated with bulbs were better than the origin of the seeds. The treatment of the two types of liquid biofertilizer produces tangible growth and yield, but there was no significant difference between the two kinds of liquid organic fertilizer.

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Growth and yield response of shallot (Allium ascalonicum L. var. Tuktuk) from different source materials applied with liquid biofertilizers

One of approaches to maintain the yield stability of the lowland tropical wheat is the use of secondary traits in the selection process. The identification of these characters requires a statistical approach in the form of genetic parameter analysis and multivariate analysis. The objective of this study was to determine the secondary traits of adaptive wheat mutants in the lowlands through the use of genetic parameters and multivariate analysis on the parameters. The study consisted of three field trials conducted in three different regencies, namely Jeneponto (135 m above sea level (asl)), Maros (100 m asl) and Bantaeng (125 m asl). The study used a nested design, where replications were nested in the environments. The genotype factors consisted of 20 genotypes repeated three times. 11 characters were observed including vegetative and reproductive characters. The analysis used consisted of repeatability, correlation, cluster analysis, principal component analysis, factor analysis, and cross print analysis. The overall results of the analysis indicate that the number of productive tillers is the main secondary trait for the selection of adaptive wheat in the lowlands. The character can be recommended for selection criteria in testing wheat lines in the lowlands to make an effective selection.

Genetic Parameters and Multivariate Analysis to Determine Secondary Traits in Selecting Wheat Mutant Adaptive on Tropical Lowlands

The main constraint on rice cultivation in the Mediterranean area is the limited irrigation and its large water consumption. In addition, rice is very sensitive to drought conditions because of drought stress on morpho-physiological traits and yield reduction. The application of salicylic acid (SA) has been noticed to be very effective in alleviating the adverse effects of drought stress on rice. The current investigation was conducted as a split-split arrangement under a randomized complete block design with two lowland rice cultivars (Giza177 and Giza179) and SA as a foliar application at four concentrations (0, 400, 700, and 1000 µM) under normal and drought conditions. The results showed that plant growth, leaf photosynthetic pigments, yields, and the most studied traits were significantly affected by irrigation (I), cultivar (C), and SA concentration (p ≤ 0.05 or 0.01). The interaction effect of I × C × SA was only significant on the carotenoids content (p ≤ 0.05). The reduction in grain yield and most studied traits was more pronounced under drought conditions. The Giza179 proved to be a drought-tolerant cultivar under all SA concentrations under drought conditions, while Giza177 was a drought-sensitive cultivar. The application of 700 µM SA gave the best grain yield in both rice cultivars under drought conditions compared to other SA concentrations. Grain yield for normal irrigation (Yp) and drought stress (Ys) conditions were highly positively correlated with indices of the mean productivity (MP), geometric mean productivity (GMP), stress tolerance index (STI), yield index (YI), yield stability index (YSI), drought resistance index (DI), harmonic mean (HM), and golden mean (GOL). While they are highly negatively correlated with the indices of the stress susceptibility index (SSI), tolerance index (TOL), yield reduction ratio (YR), stress susceptibility percentage index (SSPI), and abiotic tolerance index (ATI). It could be concluded that SA, as a growth regulator, could be used to alleviate the harmful effect of inadequate water availability in soil on rice cultivars as well as to improve the growth, water productivity, and grain yield.

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Exogenously Applied Salicylic Acid Boosts Morpho-Physiological Traits, Yield, and Water Productivity of Lowland Rice under Normal and Deficit Irrigation

A study was conducted with the aim of selecting synthetic maize genotypes tolerant to drought. The study was conducted at Experimental Farm, Faculty of Agriculture, Hasanuddin University from July to November 2017. The experiment was carried out in the form of Split Plot Design with irrigation as main plot and genotypes as subplot. Two irrigation conditions were applied to create normal and drought condition. Six synthetic maize genotypes and three comparative varieties (Bisma, Lamuru, and Sukmaraga) were used. The results showed that the genotypes Syn 2-15, and Syn 2-16 were the synthetic maize genotypes which produced higher yield and were classified as medium tolerant (MT) to drought with productivity of 6 t.ha−1 and 6.48 t.ha−1, respectively. These yields were significantly different from the varieties of Bisma and Sukmaraga in drought stress conditions. Therefore it can be concluded that adaptive synthetic maize genotypes that are provided a potential yield of ≥ 6.0 t.ha−1 in environmental stresses were Syn2-15, and Syn2-16.

https://iopscience.iop.org/article/10.1088/1755-1315/235/1/012027/pdf

Selection of various synthetic Maize (Zea mays L.) genotypes on drought stress condition

Assessment and selection of M3 generation of wheat mutants adaptive in lowland

. Sudewi S, Ala A, Baharuddin, Farid M. 2020. The isolation, characterization endophytic bacteria on from roots of local rice plant Kamba in Central Sulawesi, Indonesia. Biodiversitas 21: 1614-1624. The local Kamba rice plants are cultivated by the Bada Valley people for generations by obtaining seeds from previous crops. This rice plant is able to survive even though it is cultivated with traditional cultivation techniques. The ability to survive is suspected because this local rice plant is associated with endophytic bacteria through a variety of mechanisms including being able to produce the IAA hormone and its ability to dissolve phosphate. This study aims to find endophytic bacteria that have the potential to produce IAA and phosphate solvents through morphological and physiological characterization (Gram reaction test with 3% KOH, catalase test, hypersensitivity reaction and hemolysis activity), screening of IAA-producing bacteria qualitative and quantitative, phosphate dissolution activity on Pikovskaya medium, and quantitative using a spectrophotometer. Endophytic bacteria isolates from Gintu give the highest yields in IAA production and phosphate solubility activity, namely RKGU11 and RKGU6, respectively at 4,905 mg L-1 and 10.984 mg L-1 so that it has the potential to be used as a candidate for biofertilizer agents in developing effective sustainable agriculture, respectively, efficient and environmentally friendly.

https://smujo.id/biodiv/article/download/5398/3805

The isolation, characterization endophytic bacteria on indole-3-acetic acid-producing and phosphate solubilizing from roots of local rice plant Kamba in Bada Valley, Central Sulawesi

Muh. Farid

Papers

Multivariate analysis to determine secondary characters in selecting adaptive hybrid corn lines under drought stress

Genetic Parameters and Multivariate Analysis to Determine Secondary Traits in Selecting Wheat Mutant Adaptive on Tropical Lowlands

Selection of various synthetic Maize (Zea mays L.) genotypes on drought stress condition

Assessment and selection of M3 generation of wheat mutants adaptive in lowland

The isolation, characterization endophytic bacteria on indole-3-acetic acid-producing and phosphate solubilizing from roots of local rice plant Kamba in Bada Valley, Central Sulawesi