An indirect ELISA for the detection of japanese quail IgG specific to Newcastle disease virus (NDV) was developed The secondary anti-quail IgG was produced in Balb/c mice, by inoculating Freund's complete adjuvant emulsified japanese quail-IgG extract The purification of IgG was achieved using the caprilic acid method The ELISA was compared to the haemagglutination-inhibition (HI) test for antibodies to NDV ELISA cut-off point was established through TG-ROC analysis Total correlation was observed between the ELISA and the HI, being the ELISA efficient in the identification of positive and negative sera, with high sensitivity and specificity (100%) These results validate the use of the indirect ELISA as an alternative for the detection of NDV-specific IgG in japanese quail sera, with the advantage of high sensitivity and automation

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Indirect ELISA for the detection of IgG specific to Newcastle disease virus in quail serum

The immunogenicity of the intermediate infectious bursal disease (IBD) vaccine virus (D- 78), which commonly used in Sudan, was determined in broiler chicks in this study. The vaccine was employed via three routes of application namely aerosol, intranasal and drinking water. Both agar gel precipitation test (AGPT) and an indirect enzyme linked immunosorbent assay (ELISA) were used to detect the levels of antibody (Ab) responses in sera of vaccinated chicks. The results obtained showed that higher (P>0.05) levels of Abs were noted when the vaccine administered via aerosol route as compared to the intranasal and drinking water. The variation in the Ab levels among the chicks vaccinated with either the intranasal and drinking water using both tests was not significant ((P< 0.05). Following challenge of vaccinated chicks, the protection rates noted are correlated to the levels of Abs elicited. In conclusion, to achieve higher and protective immune status in chicks is recommended to apply the intermediate IBDV (D78) vaccine strain in broiler chicks via the aerosol route. AGPT can be used as a rapid qualitative test to determine the vaccine take among the chicks whereas ELISA should be used quantitatively to determine the levels of Ab responses in vaccinated chicks.

N Kalita

Papers

Correlation of HI and ELISA tests for detection of Newcastle disease virus antibodies

Comparative evaluation of ELISA and agar gel precipitation test for detection of infectious bursal disease viral antigens