Current status of embryo technologies in sheep and goat.

A review on reproductive biotechnologies for conservation of endangered mammalian species.

Freezing ovarian cortex is a new option to preserve the fertility of young patients undergoing cancer treatment or in women facing premature menopause. However, the best way to use this banked tissue remains unclear. The function of heterotopic and orthotopic autografts of frozen-thawed ovarian cortex of sheep was compared in the present study. Fresh and frozen-thawed fragments of ovarian cortex were autografted on the uterine horn of six ewes (orthotopic grafts) and under the skin of the belly in nine ewes (heterotopic grafts). In both orthotopic and heterotopic grafts, the resumption of follicular growth and ovulation was monitored. In orthotopically grafted ewes, fertility was recorded. Oocytes from both types of grafts were collected, matured and fertilized in vitro. In both fresh and frozen-thawed grafts follicular growth resumed normally; preantral and antral follicles were first detectable 4 and 10 weeks respectively following grafting but only 5% of the primordial follicles appeared to have survived. This confirms that grafting procedures are more deleterious for follicle survival than cryopreservation. Although ovulation resumed in most ewes, none of the ewes grafted orthotopically became pregnant at a synchronized mating. Seven months following grafting, oocytes could be collected from heterotopic and orthotopic grafts, matured and some of them fertilized, but none developed to the blastocyst stage. Heterotopic grafting may be an alternative to orthotopic grafting to preserve fertility provided follicle survival in the grafts is markedly improved.

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Orthotopic and heterotopic autografts of frozen-thawed ovarian cortex in sheep

Advanced assisted reproduction technologies (ART) in goats

Advancements in cryopreservation of domestic animal embryos.

Effect of growth factors, EGF and IGF-I, and estradiol on in vitro maturation of sheep oocytes

Comparative results of in vitro and in vivo survival of vitrified in vitro produced goat and sheep embryos

https://wcentre.tours.inra.fr/prc/internet/historique/cv/mermillod/LocatelliTherio2005_1729.pdf

N. Poulin

Papers

Current status of embryo technologies in sheep and goat.

Effect of growth factors, EGF and IGF-I, and estradiol on in vitro maturation of sheep oocytes

Comparative results of in vitro and in vivo survival of vitrified in vitro produced goat and sheep embryos

Successful use of oviduct epithelial cell coculture for in vitro production of viable red deer (Cervus elaphus) embryos.

Effect of season and duration of FSH treatment on embryo production in sheep.