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Nina Costantino
Researcher at Weizmann Institute of Science
Publications - 40
Citations - 4668
Nina Costantino is an academic researcher from Weizmann Institute of Science. The author has contributed to research in topics: Recombineering & Homologous recombination. The author has an hindex of 21, co-authored 37 publications receiving 4270 citations.
Papers
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Journal ArticleDOI
Recombineering: Genetic Engineering in Escherichia coli Using Homologous Recombination
TL;DR: The recombineering technology of recombineers as mentioned in this paper allows DNA sequences to be inserted or deleted without regard to the location of restriction sites and can also be used in combination with CRISPR/Cas targeting systems.
Posted ContentDOI
Enhancement of RecET-mediated in vivo linear DNA assembly by a xonA mutation
TL;DR: Deletion of the E. coli xonA gene, encoding Exonuclease I, a 3’→5’ single-strand DNA ex onuclease, substantially improves the efficiency of in vivo linear DNA assembly for both systems and provides a significant improvement to previously reported in vivolinear DNA assembly technologies.
Journal ArticleDOI
Recombineering in Non‐Model Bacteria
TL;DR: In this article , the authors describe the applications, challenges, and factors affecting ssDNA and dsDNA recombineering in a variety of non-model bacteria, both Gramnegative and positive, and recent breakthroughs in the field.
Patent
Host cells deficient for mismatch repair
TL;DR: In this paper, a single-stranded nucleic acid molecule has been used to induce homologous recombination in a host cell comprising of a target NCA, using a singlestranded NCA molecule.
Analysis ofMutations intheninRRegion ofBacteriophage Lambda ThatBypass a Requirement forLambdaN Antitermination
Nina Costantino,Donald L. Court +1 more
TL;DR: Analysis of the bypmutation by using promoter detection vectors, DNA sequencing, and S1nuclease analysis showed that it created a new promoter that transcribed geneQ, and the second mutation analyzed was thedeletion nin3, thereby allowing thephagetobeN independent andtogrowinhosts defective forNusantitermination factors.