The present invention is aimed to provide a method for producing a polyester with reduced CD content and elution amount, in which even after the production method, a reduction in physical properties of the polyester is small, and furthermore, a polyester having good moldability can be produced, and the present invention is concerned with a method for producing a polyester including an esterification reaction step of allowing an aliphatic diol and an aliphatic dicarboxylic acid to react with each other; a step of pelletizing a polyester obtained through the esterification reaction step; and a contact treatment step of bringing the obtained polyester pellets into contact with a mixed solution containing ethanol and water, wherein the mixed solution contains water in an amount of 10% by mass or more and not more than 99% by mass relative to the whole of the mixed solution.

Method for producing polyester

The present invention provides a method for producing a xanthophyll from a photosynthetic microalga, which includes inoculating a photosynthetic microalga containing a xanthophyll, preferably an encysted microalga, into a nutrient medium to grow the microalga; and encysting the grown microalga, by a single-step culture method in which the growth step and the encystment step are performed continuously using a nutrient medium having a low nitrogen source concentration, or by a two-step culture method in which the microalga is grown in a nutrient medium having a high nitrogen source concentration and then transferred to an encystment medium.

Method of producing xanthophyll

An encysted green alga is cultivated in a nutrient medium with a supply of carbon dioxide under irradiation with light at a photosynthetically active photon flux input of 25000 µmol-photon/m3/s or more, so that a green alga containing astaxanthin in an amount of 700 pg/cell or more, a dry product of a green alga containing astaxanthin at a concentration of 5 wt% or more, and a culture suspension of a green alga containing astaxanthin at a concentration of 150 mg/L culture suspension is obtained. A green alga belonging to the genus Haematococcus is preferable.

Green algae having a high astaxanthin content and method for producing the same

A method for preserving xanthophyll in algal cells comprises the steps of cultivating algal cells containing xanthophyll and then collecting the algal cells; and drying the collected algal cells so that they have a moisture content of 3 wt% or less Preferably, drying is performed using spray drying and under drying conditions such that the temperature of air discharged in a spray drier is 125°C or more In such dry algal cells, xanthophyll can be preserved stably for a long term

Method for preserving xanthophyll in algal cells

A system of quantitatively determining a biomolecule, which has: allowing fluorescent silica particles capable of emitting fluorescence detectable by a flow cytometer to capture a target biomolecule fluorescent-labelled for quantitative determination; detecting the fluorescence emitted from the fluorescent silica particles themselves by using the flow cytometer; and measuring the intensity of the fluorescence of the labelled target biomolecule, thereby quantitatively determining the target biomolecule.

System and method of quantitatively determining a biomolecule, system and method of detecting and separating a cell by flow cytometry, and fluorescent silica particles for use in the same, and kit comprising plural kinds of the silica particles in combination

PROBLEM TO BE SOLVED: To provide bacteria of the genus Moorella which are available for wide uses as a host cell, and a primer which is available upon creation of bacteria of the genus Moorella which are available for wide uses as a host cell. SOLUTION: Disclosed are bacteria of the genus Moorella having features that genes on chromosomes are deleted or destroyed by homologous recombination to exhibit auxotrophy, and a specific primer which amplifies a region adjacent to a gene encoding orotate phosphoribosyltransferase. COPYRIGHT: (C)2010,JPO&INPIT

Bacteria of genus moorella and primer

PROBLEM TO BE SOLVED: To provide a method for efficiently producing an astaxanthin-containing lipid with which a method for culturing a green alga Haematococcus pluvialis is improved and a cyst is thereby formed and matured under dark conditions SOLUTION: A method for producing an algal body of the green alga Haematococcus pluvialis in which astaxanthin is accumulated in an amount of ≥40-700 pg/cell in the cyst obtained by culturing the green alga Haematococcus pluvialis under conditions of 001-05 vvm relative volume of ventilation in the dark Furthermore, there is provided the method for producing the astaxanthin-containing lipid consisting essentially of a component selected from the group consisting of free type astaxanthin, an astaxanthin fatty acid monoester, an astaxanthin fatty acid diester and a mixture thereof The method is characterized by affording the astaxanthin-containing substance from the algal body COPYRIGHT: (C)2004,JPO

Method for production of astaxanthin-containing lipid

PROBLEM TO BE SOLVED: To provide a method for easily and efficiently screening a cell producing an antioxidation substance at high production rate. SOLUTION: A cell group is subjected to mutation, treated with a redox fluorescent indicator emitting fluorescence by the oxidization with an active oxygen species and irradiated with excitation light. The fluorescence emitted by the fluorescent indicator in the cell is detected by an optical detector and cells having relatively low fluorescent intensity are collected by a fractionation mechanism. Even a mutant developing at an extremely low frequency can be efficiently selected by the process. COPYRIGHT: (C)2003,JPO

Method for screening cell producing antioxidation substance at high rate

PURPOSE:To obtain a cultivation apparatus for a gaseous substrate, containing a membrane filter having attached and immobilized microorganisms in the interior of a container having a hermetically sealable planar bottom, supply and discharge ports for a gas as well as supply port for a liquid and capable of accurately controlling cultivation. CONSTITUTION:A cultivation apparatus obtained by providing a flange 32 joining to a cover (B) at the top end part of a bottomed cylindrical body 31 having a horizontal bottom to form the body (A) of the apparatus, providing a discharge port 33 for a liquid at the end of the cylindrical body 31, supply port 37 for a gas, gas supply port 36 and gas discharged port 38 at the cover (B) and containing a membrane filter having attached and immobilized microorganisms in the bottom of the body (A).

Cultivation apparatus for gaseous substrate

PURPOSE:To produce acetic acid from inexpensive materials by using Clostridium thermoaceticum. CONSTITUTION:Clostridium thermoaceticum is cultured in a compound substrate composed mainly of glucose, methanol and carbon dioxide gas source in absolute anaerobic state, separating a liquid component from the cultured liquid by solid-liquid separation and producing acetic acid from the separated liquid.

Nishio Naomichi

Papers

Bacteria of genus moorella and primer

Method for production of astaxanthin-containing lipid

Method for screening cell producing antioxidation substance at high rate

Cultivation apparatus for gaseous substrate

Production of acetic acid