Many biological applications require the segmentation of cell bodies, membranes and nuclei from microscopy images. Deep learning has enabled great progress on this problem, but current methods are specialized for images that have large training datasets. Here we introduce a generalist, deep learning-based segmentation method called Cellpose, which can precisely segment cells from a wide range of image types and does not require model retraining or parameter adjustments. Cellpose was trained on a new dataset of highly varied images of cells, containing over 70,000 segmented objects. We also demonstrate a three-dimensional (3D) extension of Cellpose that reuses the two-dimensional (2D) model and does not require 3D-labeled data. To support community contributions to the training data, we developed software for manual labeling and for curation of the automated results. Periodically retraining the model on the community-contributed data will ensure that Cellpose improves constantly.

Cellpose: a generalist algorithm for cellular segmentation

Towards the development of realistic botnet dataset in the Internet of Things for network forensic analytics: Bot-IoT dataset

Two-photon microscopy of calcium-dependent sensors has enabled unprecedented recordings from vast populations of neurons. While the sensors and microscopes have matured over several generations of development, computational methods to process the resulting movies remain inefficient and can give results that are hard to interpret. Here we introduce Suite2p: a fast, accurate and complete pipeline that registers raw movies, detects active cells, extracts their calcium traces and infers their spike times. Suite2p runs on standard workstations, operates faster than real time, and recovers ~2 times more cells than the previous state-of-the-art method. Its low computational load allows routine detection of ~10,000 cells simultaneously with standard two-photon resonant-scanning microscopes. Recordings at this scale promise to reveal the fine structure of activity in large populations of neurons or large populations of subcellular structures such as synaptic boutons.

/pdf/suite2p-beyond-10-000-neurons-with-standard-two-photon-3042enp0y5.pdf

Suite2p: beyond 10,000 neurons with standard two-photon microscopy

The Internet of Things (IoT) integrates billions of smart devices that can communicate with one another with minimal human intervention. IoT is one of the fastest developing fields in the history of computing, with an estimated 50 billion devices by the end of 2020. However, the crosscutting nature of IoT systems and the multidisciplinary components involved in the deployment of such systems have introduced new security challenges. Implementing security measures, such as encryption, authentication, access control, network and application security for IoT devices and their inherent vulnerabilities is ineffective. Therefore, existing security methods should be enhanced to effectively secure the IoT ecosystem. Machine learning and deep learning (ML/DL) have advanced considerably over the last few years, and machine intelligence has transitioned from laboratory novelty to practical machinery in several important applications. Consequently, ML/DL methods are important in transforming the security of IoT systems from merely facilitating secure communication between devices to security-based intelligence systems. The goal of this work is to provide a comprehensive survey of ML methods and recent advances in DL methods that can be used to develop enhanced security methods for IoT systems. IoT security threats that are related to inherent or newly introduced threats are presented, and various potential IoT system attack surfaces and the possible threats related to each surface are discussed. We then thoroughly review ML/DL methods for IoT security and present the opportunities, advantages and shortcomings of each method. We discuss the opportunities and challenges involved in applying ML/DL to IoT security. These opportunities and challenges can serve as potential future research directions.

A Survey of Machine and Deep Learning Methods for Internet of Things (IoT) Security

The Internet of Things (IoT) refers to the interconnection of smart devices to collect data and make intelligent decisions. However, a lack of intrinsic security measures makes IoT vulnerable to privacy and security threats. With its "security by design," Blockchain (BC) can help in addressing major security requirements in IoT. BC capabilities like immutability, transparency, auditability, data encryption and operational resilience can help solve most architectural shortcomings of IoT. This article presents a comprehensive survey on BC and IoT integration. The objective of this paper is to analyze the current research trends on the usage of BC-related approaches and technologies in an IoT context. This paper presents the following novelties, with respect to related work: (i) it covers different application domains, organizing the available literature according to this categorization, (ii) it introduces two usage patterns, i.e., device manipulation and data management (open marketplace solution), and (iii) it reports on the development level of some of the presented solutions. We also analyze the main challenges faced by the research community in the smooth integration of BC and IoT, and point out the main open issues and future research directions. Last but not least, we also present a survey about novel uses of BC in the machine economy.

https://www.mdpi.com/1424-8220/18/8/2575/pdf

Blockchain and IoT Integration: A Systematic Survey.

An increasing number of Internet of Things (IoT) devices are connecting to the Internet, yet many of these devices are fundamentally insecure, exposing the Internet to a variety of attacks. Botnets such as Mirai have used insecure consumer IoT devices to conduct distributed denial of service (DDoS) attacks on critical Internet infrastructure. This motivates the development of new techniques to automatically detect consumer IoT attack traffic. In this paper, we demonstrate that using IoT-specific network behaviors (e.g., limited number of endpoints and regular time intervals between packets) to inform feature selection can result in high accuracy DDoS detection in IoT network traffic with a variety of machine learning algorithms, including neural networks. These results indicate that home gateway routers or other network middleboxes could automatically detect local IoT device sources of DDoS attacks using low-cost machine learning algorithms and traffic data that is flow-based and protocol-agnostic.

/pdf/machine-learning-ddos-detection-for-consumer-internet-of-2atsfu6zta.pdf

Machine Learning DDoS Detection for Consumer Internet of Things Devices

Smart home Internet of Things (IoT) devices are rapidly increasing in popularity, with more households including Internet-connected devices that continuously monitor user activities. In this study, we conduct eleven semi-structured interviews with smart home owners, investigating their reasons for purchasing IoT devices, perceptions of smart home privacy risks, and actions taken to protect their privacy from those external to the home who create, manage, track, or regulate IoT devices and/or their data. We note several recurring themes. First, users' desires for convenience and connectedness dictate their privacy-related behaviors for dealing with external entities, such as device manufacturers, Internet Service Providers, governments, and advertisers. Second, user opinions about external entities collecting smart home data depend on perceived benefit from these entities. Third, users trust IoT device manufacturers to protect their privacy but do not verify that these protections are in place. Fourth, users are unaware of privacy risks from inference algorithms operating on data from non-audio/visual devices. These findings motivate several recommendations for device designers, researchers, and industry standards to better match device privacy features to the expectations and preferences of smart home owners.

https://dl.acm.org/doi/pdf/10.1145/3274469

User Perceptions of Smart Home IoT Privacy

The increasing popularity of specialized Internet-connected devices and appliances, dubbed the Internet-of-Things (IoT), promises both new conveniences and new privacy concerns Unlike traditional web browsers, many IoT devices have always-on sensors that constantly monitor fine-grained details of users' physical environments and influence the devices' network communications Passive network observers, such as Internet service providers, could potentially analyze IoT network traffic to infer sensitive details about users Here, we examine four IoT smart home devices (a Sense sleep monitor, a Nest Cam Indoor security camera, a WeMo switch, and an Amazon Echo) and find that their network traffic rates can reveal potentially sensitive user interactions even when the traffic is encrypted These results indicate that a technological solution is needed to protect IoT device owner privacy, and that IoT-specific concerns must be considered in the ongoing policy debate around ISP data collection and usage

A Smart Home is No Castle: Privacy Vulnerabilities of Encrypted IoT Traffic

The growing market for smart home IoT devices promises new conveniences for consumers while presenting new challenges for preserving privacy within the home. Many smart home devices have always-on sensors that capture users' offline activities in their living spaces and transmit information about these activities on the Internet. In this paper, we demonstrate that an ISP or other network observer can infer privacy sensitive in-home activities by analyzing Internet traffic from smart homes containing commercially-available IoT devices even when the devices use encryption. We evaluate several strategies for mitigating the privacy risks associated with smart home device traffic, including blocking, tunneling, and rate-shaping. Our experiments show that traffic shaping can effectively and practically mitigate many privacy risks associated with smart home IoT devices. We find that 40KB/s extra bandwidth usage is enough to protect user activities from a passive network adversary. This bandwidth cost is well within the Internet speed limits and data caps for many smart homes.

Noah Apthorpe

Papers

Machine Learning DDoS Detection for Consumer Internet of Things Devices

User Perceptions of Smart Home IoT Privacy

A Smart Home is No Castle: Privacy Vulnerabilities of Encrypted IoT Traffic

Spying on the Smart Home: Privacy Attacks and Defenses on Encrypted IoT Traffic.

User Perceptions of Smart Home IoT Privacy