Showing papers by "Norton D. Zinder published in 1967"
TL;DR: Two amber mutations in the coat gene of the phage f2 have been shown to occur at the sites specifying amino acid residue 6 and residue 70 in the intact coat protein, where the translation of the amber codon as a chain termination signal leads to the release of the coat protein fragment with its carboxy-terminus free of any attached tRNA.
75 citations
61 citations
TL;DR: The data support the hypothesis that the genes on this RNA are translated sequentially in vitro, and that the translation of the non-coat genes is contingent upon thetranslation of at least the codon for site 6 of the coat protein gene.
57 citations
TL;DR: A new type of host-dependent mutant, azure mutant, of bacteriophage f2 has been isolated and it is proposed that the azure codon is the same as the amber codon and that restriction results from improper termination of protein chains of the phage RNA polymerase.
Abstract: A new type of host-dependent mutant, azure mutant, of bacteriophage f2 has been isolated. Growth of these mutants was restricted specifically by amber suppressor genes in the host bacteria. Restriction of the formation of infective centers by different bacterial suppressor genes was 98 percent, 90 percent, and 70 percent with Su-3, Su-1, and Su-2 genes, respectively. Restriction, like suppression, was the dominant phenotype. The block in growth of the mutants occurred in an early stage of the infection cycle. Once infection was established, however, an infected cell produced approximately the same number of progeny phage as a cell without the suppressor genes did. It is proposed that the azure codon is the same as the amber codon (uracil, adenine, guanine) and that restriction results from improper termination of protein chains of the phage RNA polymerase. Similar mutants may exist in other systems.
35 citations