Showing papers by "Norton D. Zinder published in 1972"
TL;DR: A series of four-point crosses was devised and carried out in such a way that the progeny of single mixedly infected cells in which recombination events had taken place could be analyzed.
178 citations
TL;DR: The results indicate that Circular replicative forms are broken once to yield full-length linear molecules (RF III), which are less susceptible than RF I to endonuclease R-B to B-restriction.
Abstract: We studied the cleavage of the replicative-form DNA (RF I) of bacteriophage f1 and its SB mutants by purified restriction endonuclease of E. coli B. The results indicate that: (i) Circular replicative forms are broken once to yield full-length linear molecules (RF III). Such linear molecules are less susceptible than RF I to endonuclease R-B. (ii) The genetic sites (SB sites) that confer on the DNA susceptibility to B-restriction are not the actual sites of cleavage. The number of possible cleavage sites is larger than the number of SB sites. We conclude this because an RF III molecule produced by endonuclease R-B from RF I of a mutant that has only one SB site can be circularized by denaturation and renaturation. (iii) The SB site is not modified when the DNA is cleaved, since an SB site can be used repeatedly by endonuclease R-B; the RF III described in ii can be cleaved by the same enzyme after denaturation and renaturation.
80 citations
TL;DR: The results suggest that the f2 replicase is capable of limited in vitro chain termination and reinitiation of RNA synthesis, and homologous to f2 RNAs by annealing criteria.
13 citations
TL;DR: Complex substrate saturation kinetics are observed for GTP in both replicase and poly(G) polymerase reactions, and double reciprocal plots are relatively linear at high substrate concentrations, but markedly non-linear at low substrate concentrations.
6 citations