Showing papers by "Owen T. Gorman published in 1998"
TL;DR: The branching topology of the recent equine lineage indicates recent introduction of the NS, M, and PB2 genes into horses from avian sources by genetic reassortment.
80 citations
TL;DR: Methods for the cryopreservation of sperm were developed, the influence of various factors on motility of thawed sperm was evaluated, and the effect on fertilization of cooling rate and the addition of caffeine was examined.
Abstract: The endangered razorback sucker Xyrauchen texanus is endemic to the Colorado River system in western North America and is threatened with extinction because of limited recruitment. To assist in management and recovery efforts, we developed methods for the cryopreservation of sperm, evaluated the influence of various factors on motility of thawed sperm, and examined the effect on fertilization of cooling rate and the addition of caffeine. Sperm samples cryopreserved with 10% methanol (MeOH) had significantly higher postthaw motility than did samples preserved with 5% or 20% MeOH or with 5% or 10% dimethyl sulfoxide, N, N-dimethylacetamide, glycerol, propylene glycol, or ethylene glycol. Sperm samples cryopreserved in 0.5-mL and 2.5-mL straws had significantly higher postthaw motility than did samples cryopreserved in 0.25-mL straws. Exposure to 10% MeOH for up to 30 min did not significantly influence sperm motility before freezing or after thawing. Cooling rate (−21°C/min or −91°C/min) did not si...
58 citations