P
P.H. Pouwels
Publications - 22
Citations - 1940
P.H. Pouwels is an academic researcher. The author has contributed to research in topics: Gene & Transformation (genetics). The author has an hindex of 13, co-authored 22 publications receiving 1898 citations.
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Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.
TL;DR: A new, heterologous, dominant marker for selection of Aspergillus transformants is described, based on the Escherichia coli hygromycin B (HmB) phosphotransferase gene (hph), which confers HmB resistance to As pergillus species.
Journal ArticleDOI
Development of a homologous transformation system for Aspergillus niger based on the pyrG gene
W. van Hartingsveldt,I. E. Mattern,C. M. J. Van Zeijl,P.H. Pouwels,C.A.M.J.J. van den Hondel +4 more
TL;DR: The pyrG transformation system was used for the introduction of a non-selectable gene into A. niger through the use of an orotidine-5′-phosphate decarboxylase deficient mutant (pyrG) and a vector, pAB4-1, which contains the functional A.Niger pyr G gene as a selection marker.
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Intracellular and extracellular production of proteins in Aspergillus under the control of expression signals of the highly expressed Aspergillus nidulans gpdA gene.
TL;DR: The expression in Aspergillus is described of genes, coding for intracellular and extracellular proteins controlled by the promoter region of the constitutively and efficiently expressed glyceraldehyde-3-phosphate dehydrogenase gene (gpdA) of As pergillus nidulans.
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Gene amplification in Aspergillus nidulans by transformation with vectors containing the amdS gene
K. Wernars,Theo Goosen,L.M.J. Wennekes,Jaap Visser,C.J. Bos,H.W.J. van den Broek,R.F.M. van Gorcom,C.A.M.J.J. van den Hondel,P.H. Pouwels +8 more
TL;DR: Analysis of DNA from AmdS+ transformants of MH1277 showed that transformation had occurred by integration of vector DNA sequences into the genome, and in virtually all transformants multiple copies of the vector were present in a tandemly repeated fashion.
Journal ArticleDOI
Fusion proteins with multiple copies of the major antigenic determinant of foot-and-mouth disease virus protect both the natural host and laboratory animals.
M. P. Broekhuijsen,J. M. M. Van Rijn,A. J. M. Blom,P.H. Pouwels,Betty E. Enger-Valk,F. Brown,M. J. Francis +6 more
TL;DR: Proteins consisting of one, two or four copies of the amino acid sequence 137 to 162, which contains the major immunogenic site of VP1 of foot-and-mouth disease virus, attached to the N-terminus of beta-galactosidase have been expressed in Escherichia coli cells.