P. L. Summers

TL;DR: The protective monoclonal antibodies were directed against viral determinants that fell into at least three spatially separate families of epitopes on E-glycoprotein, the antigenicities of which were preserved after heat/detergent denaturation.

TL;DR: Five murine monoclonal antibodies reactive against the prM glycoproteins of DEN-3 and -4 were used to passively protect mice in vivo against lethal challenge with homologous and heterologous dengue virus serotypes, the first report of prM-specific Mabs that are protective in mice.

TL;DR: A recombinant baculovirus containing a 4.0-kilobase dengue virus cDNA sequence that codes for the three virus structural proteins, capsid (C) protein, premembrane (PreM)protein, and envelope glycoprotein (E) and nonstructural proteins NS1 and NS2a is constructed.

TL;DR: The entry modes of Japanese encephalitis (JE) and dengue-2 (DEN-2) viruses into C6/36 mosquito cells and of DEN-2 virus into human peripheral blood monocytes in vitro were studied and implications of the entry mode to an early process of natural, mosquito-transmitted infection are discussed.

TL;DR: A recombinant vaccinia virus containing cloned DNA sequences coding for the three structural proteins and nonstructural proteins NS1 and NS2a of dengue type 4 virus was constructed, and an immune response to the PreM and E glycoproteins was not detected.