Immunization of mice with dengue structural proteins and nonstructural protein NS1 expressed by baculovirus recombinant induces resistance to dengue virus encephalitis.
Y. M. Zhang,E P Hayes,Thomas R. McCarty,Doria R. Dubois,P. L. Summers,Kenneth H. Eckels,R M Chanock,Ching-Juh Lai +7 more
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A recombinant baculovirus containing a 4.0-kilobase dengue virus cDNA sequence that codes for the three virus structural proteins, capsid (C) protein, premembrane (PreM)protein, and envelope glycoprotein (E) and nonstructural proteins NS1 and NS2a is constructed.Abstract:
We have constructed a recombinant baculovirus containing a 4.0-kilobase dengue virus cDNA sequence that codes for the three virus structural proteins, capsid (C) protein, premembrane (PreM) protein, and envelope glycoprotein (E), and nonstructural proteins NS1 and NS2a. Infection of cultured Spodoptera frugiperda cells with this recombinant virus resulted in the production of E and NS1 proteins that were similar in size to the corresponding viral proteins expressed in dengue virus-infected simian cells. Other dengue virus-encoded proteins such as PreM and C were also synthesized. Rabbits immunized with the dengue virus protein products of the recombinant virus developed antibodies to PreM, E, and NS1, although the titers were low, especially to PreM and E. Nevertheless, the dengue virus antigens produced by the recombinant virus induced resistance in mice to fatal dengue encephalitis.read more
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References
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A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures.
Summers,G.E. Smith +1 more
Journal ArticleDOI
Pathogenesis of dengue: challenges to molecular biology
TL;DR: This work has identified a severe syndrome, dengue hemorrhagic fever/dengue shock syndrome, in Southeast Asian children, which recently has also been identified in children infected with the virus in Puerto Rico.
Journal ArticleDOI
Trends in the development of baculovirus expression vectors
Verne A. Luckow,Max D. Summers +1 more
TL;DR: The current status and potential use of baculovirus vectors for the expression of foreign genes in insect cells are described and are contributing to understanding the molecular biology of gene and protein function and regulation in both vertebrate and insect systems.
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Production of human beta interferon in insect cells infected with a baculovirus expression vector.
TL;DR: Results demonstrate that AcNPV should be suitable for use as a eucaryotic expression vector for the production of products from cloned genes.
Journal ArticleDOI
Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques.
TL;DR: Each protocol was tested by constructing vaccinia virus recombinants that express hepatitis B virus surface antigen by constructing plasmid coexpression vector that directs the insertion of a foreign gene of interest together with the Escherichia coli beta-galactosidase (beta gal) gene into the thymidine kinase (TK) locus of the vaccinations genome.