P
Pål Nyrén
Researcher at Royal Institute of Technology
Publications - 54
Citations - 5412
Pål Nyrén is an academic researcher from Royal Institute of Technology. The author has contributed to research in topics: Pyrosequencing & DNA sequencing. The author has an hindex of 26, co-authored 54 publications receiving 5263 citations.
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Journal ArticleDOI
A Sequencing Method Based on Real-Time Pyrophosphate
Journal ArticleDOI
Comparative analysis of human gut microbiota by barcoded pyrosequencing.
Anders F. Andersson,Anders F. Andersson,Mathilda Lindberg,Mathilda Lindberg,Hedvig E. Jakobsson,Hedvig E. Jakobsson,Fredrik Bäckhed,Pål Nyrén,Lars Engstrand,Lars Engstrand +9 more
TL;DR: This work has employed 454-pyrosequencing of a hyper-variable region of the 16S rRNA gene in combination with sample-specific barcode sequences which enables parallel in-depth analysis of hundreds of samples with limited sample processing, and demonstrated that the method correctly describes microbial communities down to phylotypes below the genus level.
Journal ArticleDOI
Single-Nucleotide Polymorphism Analysis by Pyrosequencing
Afshin Ahmadian,Baback Gharizadeh,Anna C. Gustafsson,Fredrik Sterky,Pål Nyrén,Mathias Uhlén,Joakim Lundeberg +6 more
TL;DR: It is demonstrated that typing of SNPs can efficiently be performed by pyrosequencing using an automated system for parallel analysis of 96 samples in approximately 5 min, suitable for large-scale screening and typing ofSNPs.
Journal ArticleDOI
Solid phase DNA minisequencing by an enzymatic luminometric inorganic pyrophosphate detection assay.
TL;DR: It is shown that the concept can be used for sequencing of single bases as well as stepwise analysis of several subsequent bases for non-radioactive detection of single base changes without the need for electrophoresis.
Journal ArticleDOI
Analyses of Secondary Structures in DNA by Pyrosequencing
TL;DR: A part of the polymorphic pertussis toxin promoter region in five different Bordetella species was successfully resolved by the new technique, and the obtained sequence data revealed four related palindromic sequences.