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Afshin Ahmadian
Researcher at Royal Institute of Technology
Publications - 76
Citations - 3376
Afshin Ahmadian is an academic researcher from Royal Institute of Technology. The author has contributed to research in topics: Gene & Pyrosequencing. The author has an hindex of 29, co-authored 75 publications receiving 3268 citations.
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Journal ArticleDOI
Single-Nucleotide Polymorphism Analysis by Pyrosequencing
Afshin Ahmadian,Baback Gharizadeh,Anna C. Gustafsson,Fredrik Sterky,Pål Nyrén,Mathias Uhlén,Joakim Lundeberg +6 more
TL;DR: It is demonstrated that typing of SNPs can efficiently be performed by pyrosequencing using an automated system for parallel analysis of 96 samples in approximately 5 min, suitable for large-scale screening and typing ofSNPs.
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Pyrosequencing: History, biochemistry and future
TL;DR: This work has shown that the read length of the pyrosequencing technique is limited to analysis of short DNA sequences exemplified by single-nucleotide polymorphism analysis and genotyping, and the importance of high-throughput DNA analysis techniques is significant.
Journal ArticleDOI
Generations of sequencing technologies.
TL;DR: Current massively parallel, state-of-the-art systems are providing significantly improved throughput over Sanger systems and future single-molecule approaches will continue the exponential improvements in the field.
Journal Article
Human epidermal cancer and accompanying precursors have identical p53 mutations different from p53 mutations in adjacent areas of clonally expanded non-neoplastic keratinocytes.
Zhi-Ping Ren,A Hedrum,Fredrik Pontén,Monica Nistér,Afshin Ahmadian,Joakim Lundeberg,Mathias Uhlén,Jan Pontén +7 more
TL;DR: The findings indicate that a large fraction of keratinocytes in sun-exposed human skin carry mutations of p53 and suggest that at least two options exist for such cells (i) innocuous clonal expansion with preserved morphology and normal differentiation or (ii) malignant transformation with the p53 mutation as an early event.
Journal ArticleDOI
PATCHED and p53 gene alterations in sporadic and hereditary basal cell cancer.
Gao Ling,Afshin Ahmadian,Åsa Persson,Anne Birgitte Undén,Gijs B. Afink,Cecilia Williams,Mathias Uhlén,Rune Toftgård,Joakim Lundeberg,Fredrik Pontén +9 more
TL;DR: The high frequency and co-existence of genetic alterations in the patched and p53 genes suggest that both these genes are important in the development of basal cell cancer.