Showing papers by "Paul Digard published in 1991"
TL;DR: The results raise the possibility that there is a fundamental difference betweenalpha-like DNA polymerases and E. coli DNA polymerase I, with less distinction between 3'-5' exonuclease and polymerase functions in alpha-likeDNA polymerases.
Abstract: Most DNA polymerases are multifunctional proteins that possess both polymerizing and exonucleolytic activities. For Escherichia coli DNA polymerase I and its relatives, polymerase and exonuclease activities reside on distinct, separable domains of the same polypeptide. The catalytic subunits of the alpha-like DNA polymerase family share regions of sequence homology with the 3'-5' exonuclease active site of DNA polymerase I; in certain alpha-like DNA polymerases, these regions of homology have been shown to be important for exonuclease activity. This finding has led to the hypothesis that alpha-like DNA polymerases also contain a distinct 3'-5' exonuclease domain. We have introduced conservative substitutions into a 3'-5' exonuclease active site homology in the gene encoding herpes simplex virus DNA polymerase, an alpha-like polymerase. Two mutants were severely impaired for viral DNA replication and polymerase activity. The mutants were not detectably affected in the ability of the polymerase to interact with its accessory protein, UL42, or to colocalize in infected cell nuclei with the major viral DNA-binding protein, ICP8, suggesting that the mutation did not exert global effects on protein folding. The results raise the possibility that there is a fundamental difference between alpha-like DNA polymerases and E. coli DNA polymerase I, with less distinction between 3'-5' exonuclease and polymerase functions in alpha-like DNA polymerases.
54 citations
Patent•
21 Feb 1991TL;DR: An immunoassay for identifying an inhibitor of HSV DNA replication is described in this article, where the authors provide a DNA polymerizing complex that includes two complex members, the complex members being HSVDNA polymerase and UL42, and determine whether the potential inhibitor inhibits formation of the complex.
Abstract: An immunoassay for identifying an inhibitor of HSV DNA replication including providing a DNA polymerizing complex that includes two complex members, the complex members being HSV DNA polymerase and UL42, providing a potential inhibitor that inhibits the binding of HSV DNA polymerase to UL42, mixing the complex members in the presence of the potential inhibitor, and determining whether the potential inhibitor inhibits formation of the complex.
21 citations