Paul E. Stephens

TL;DR: The nucleotide sequence of the aceF gene, which encodes the dihydrolipoamide acetyltransferase component (E2) of the pyruvate dehydrogenase complex of Escherichia coli K12, has been determined using the dideoxy chain-termination method and supports conclusions that the acetyl transferase subunit possesses two heterologous domains.

TL;DR: The results confirm that the lpd gene is an independent gene linked to, but not part of, the ace operon that encodes the E1 and E2 components of the pyruvate dehydrogenase complex.

TL;DR: The nucleotide sequence of the sucB gene, which encodes the dihydrolipoamide succinyltransferase component (E2o) of the 2-oxoglutarate dehydrogenase complex of Escherichia coli K12, has been determined by the dideoxy chain-termination method.

TL;DR: The nucleotide sequence of a 3780-base-pair segment of DNA containing the aceE gene encoding the pyruvate dehydrogenase component (E1) of the pyrivate dehydration complex of Escherichia coli, has been determined by the dideoxy chain-termination method.

TL;DR: Three components of the pyruvate dehydrogenase complex of Escherichia coli are encoded by three linked genes, ace E, aceF and lpd, situated close to the nadC (quinolinate phosphoribosyltransferase) and aroP (general aromatic amino acid permease) genes with the gene order: nad C-aroP-aceE-aceF-lpd.