The invention discloses a method for extracting tigogenin from agave sisalana residue, belongs to the technical field of active component extraction. The method comprises the following steps of pretreatment of raw materials, enzymatic hydrolysis, fermentation treatment, centrifugal collection, purifying treatment and drying treatment. During enzymatic hydrolysis process, the tigogenin is fully separated out by a compound enzyme and the separating rate is quickened. The compound enzyme is prepared from the following raw materials: 18 to 26 parts of cellulase, 10 to 15 parts of pectinase, 1 to3 parts of glucosidase, 2 to 4 parts of proteolytic enzyme and 2 to 4 parts of amylase. During fermentation treatment process, the separating rate of the tigogenin is further improved by active mixedyeast, and the active mixed yeast is prepared from the following raw materials: 6 to 10 parts of aspergillus niger, 3 to 5 parts of neurospora, 4 to 8 parts of penicillium, 6 to 8 parts of trichodermaharzianum, 6 to 10 parts of torulaspora debrueckii and 8 to 12 parts of ethanol-extracted candida. According to the method, the problem that the extracting time is long and the yield of the tigogeninis low in the existing method for extracting tigogenin from agave sisalana residue is solved.

Method for extracting tigogenin from agave sisalana residue

The invention discloses a method for extraction of high-purity tigogenin from sisal hemp residue. The method includes pool fermentation, pressure filtration and stacking fermentation steps. The fermentation method provided by the invention enables full fermentation, reduces the impurity content of sisal hemp paste, greatly improves the yield and purity of tigogenin, also greatly utilizes the effective ingredient tigogenin in sisal hemp, and reduces waste.

Production fermentation method for sisal hemp paste in tigogenin extraction

The invention relates to a sisal hemp-plant leaf cream production method. According to the production method, sisal hemp-plant leaves are produced into fibers to obtain sisal hemp-plant leaf juice, the juice is fermented, sediments are collected, leuconostoc mesenteroides, acclimated ethyl alcohol candida and lactobacillus delbrueckii are mixed to prepare fermentation inoculants for fermenting, acclimation is performed by the aid of a gradual acclimation method, the proportion of sisal hemp-plant leaf juice in acclimation culture solution is gradually increased, and the ethyl alcohol candida and the lactobacillus delbrueckii are respectively and gradually inoculated into high-proportion culture solution from low-proportion culture solution to obtain the acclimated ethyl alcohol candida and the acclimated lactobacillus delbrueckii. According to the method, a fermentation period is shortened to 4-5 days, yield of the sisal hemp-plant leaf cream is greatly improved, diosgenin content is improved by 20-30%, and difficulties further extracting diosgenin are reduced. The method changes a traditional production mode and solves the problem that a traditional method is large in occupied area, long in settlement period, low in diosgenin content and the like.

Peng Yuande

Papers

Sisal hemp-plant leaf cream production method