Showing papers by "Peter Kille published in 1993"
TL;DR: From genomic libraries constructed for both pike and stone loach, clones were isolated containing the metallothionein genes from these two species of cadmium-tolerant fish and neither gene locus showed any homology with the glucocorticoid or interferon responsive elements that have been identified in mammalian species.
35 citations
TL;DR: Comparison with known LPL sequences from other species shows a high degree of conservation in the sequence of the protein and in the 5' untranslated region of the DNA sequence.
21 citations
TL;DR: E coli ACP can be used as a substitute for plant ACP in many assays for plant fatty acid synthetase activity; indeed it sometimes proves more active in vitro than the native plantACP as mentioned in this paper, which is useful because such large quantities of plant material are required to isolate relatively small amounts of protein.
Abstract: Acyl carrier protein (ACP) is a small (9kDa) protein which is essential for fatty acid synthesis In bacteria [ l ] and plants [2] which use type I1 dissociable fatty acid synthetases, acyl chains are esterified to ACP through the 4'-phosphopantetheine prosthetic group, and ACP acts as a carrier during fatty acid synthesis ACPs from bacteria and plants are very similar All are small acidic proteins with conserved regions particularly around the serine residue through which the prosthetic group is attached The similarity of E coli ACP to many plant ACPs is such that E coli ACP can be used as a substitute for plant ACP in many assays for plant fatty acid synthetase activity; indeed it sometimes proves more active in vitro than the native plant ACP [3] This is useful because such large quantities of plant material are required to isolate relatively small amounts of protein It is therefore easier to isolate E coli ACP E coli contains only one ACP isoform and also has the advantage of being active in many plant system, whereas plant ACPs often do not work in other plant systems, and different and specific isoforms occur in different tissues [4] It is possible to isolate 20-30mg of ACP from 200g of E coli wet cell paste in 3 days However, assaying plant fatty acid synthesis enzymes requires large quantities of ACP and therefore,
7 citations
01 Mar 1993
TL;DR: Acyl carrier protein (ACP) from E. coli was cloned using degenerate PCR and the resulting clone was sequenced and translated to give a product which had the known amino acid sequence of E. bacteria ACP.
Abstract: Acyl carrier protein (ACP) from E. coli was cloned using degenerate PCR. The resulting clone was sequenced and translated to give a product which had the known amino acid sequence of E. coli ACP. The insert obtained was cloned into various expression systems, but no overexpression was obtained and sequencing of the insert revealed the presence of mutations.
2 citations