P
Philipp J. Keller
Researcher at Howard Hughes Medical Institute
Publications - 93
Citations - 9975
Philipp J. Keller is an academic researcher from Howard Hughes Medical Institute. The author has contributed to research in topics: Light sheet fluorescence microscopy & Microscopy. The author has an hindex of 40, co-authored 91 publications receiving 8150 citations. Previous affiliations of Philipp J. Keller include Max Planck Society & Janelia Farm Research Campus.
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Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy.
TL;DR: This work developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development to derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event.
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Whole-brain functional imaging at cellular resolution using light-sheet microscopy
TL;DR: Light-sheet microscopy is used to record activity from the entire volume of the brain of the larval zebrafish in vivo at 0.8 Hz, capturing more than 80% of all neurons at single-cell resolution, demonstrating how this technique can be used to reveal functionally defined circuits across the brain.
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Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy
Philipp J. Keller,Annette D. Schmidt,Anthony Santella,Khaled Khairy,Zhirong Bao,Joachim Wittbrodt,Joachim Wittbrodt,Ernst H. K. Stelzer +7 more
TL;DR: This method discriminates the specimen-related scattered background from signal fluorescence, thereby removing out-of-focus light and optimizing the contrast of in-focus structures, and provides rapid control of the illumination pattern, exceptional imaging quality and high imaging speeds.
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Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy.
TL;DR: This work developed one-photon and multiphoton SiMView implementations and recorded cellular dynamics in entire Drosophila melanogaster embryos with 30-s temporal resolution throughout development and performed high-resolution long-term imaging of the developing nervous system and followed neuroblast cell lineages in vivo.
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A general method to fine-tune fluorophores for live-cell and in vivo imaging
Jonathan B. Grimm,Anand K. Muthusamy,Yajie Liang,Timothy A. Brown,William C. Lemon,Ronak Patel,Rongwen Lu,John J. Macklin,Philipp J. Keller,Na Ji,Luke D. Lavis +10 more
TL;DR: This work refined and extended the Janelia Fluor strategy, finding that incorporation of 3-substituted azetidine groups allows rational tuning of the spectral and chemical properties of rhodamine dyes with unprecedented precision.