L
Luke D. Lavis
Researcher at Howard Hughes Medical Institute
Publications - 165
Citations - 12703
Luke D. Lavis is an academic researcher from Howard Hughes Medical Institute. The author has contributed to research in topics: Chromatin & Fluorophore. The author has an hindex of 48, co-authored 150 publications receiving 9564 citations. Previous affiliations of Luke D. Lavis include Yale University & Janelia Farm Research Campus.
Papers
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Journal ArticleDOI
A general method to improve fluorophores for live-cell and single-molecule microscopy
Jonathan B. Grimm,Brian P. English,Jiji Chen,Joel P Slaughter,Zhengjian Zhang,Andrey Revyakin,Ronak Patel,John J. Macklin,Davide Normanno,Robert H. Singer,Timothée Lionnet,Luke D. Lavis +11 more
TL;DR: Inspired by molecular modeling, the N,N-dimethylamino substituents in tetramethylrhodamine are replaced with four-membered azetidine rings, which doubles the quantum efficiency and improves the photon yield of the dye in applications ranging from in vitro single-molecule measurements to super-resolution imaging.
Journal ArticleDOI
Bright Ideas for Chemical Biology
Luke D. Lavis,Ronald T. Raines +1 more
TL;DR: The chemical and photophysical properties of oft-used fluorophores are examined and classic and contemporary examples in which utility has been built upon these scaffolds are highlighted.
Journal ArticleDOI
Imaging dynamic and selective low-complexity domain interactions that control gene transcription
Shasha Chong,Claire Dugast-Darzacq,Zhe Liu,Peng Dong,Gina M. Dailey,Claudia Cattoglio,Alec Heckert,Sambashiva Banala,Luke D. Lavis,Xavier Darzacq,Robert Tjian +10 more
TL;DR: Live-cell single-molecule imaging revealed that TF LCDs interact to form local high-concentration hubs at both synthetic DNA arrays and endogenous genomic loci, suggesting that under physiological conditions, rapid, reversible, and selective multivalent LCD-LCD interactions occur between TFs and the RNA Pol II machinery to activate transcription.
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A general method to fine-tune fluorophores for live-cell and in vivo imaging
Jonathan B. Grimm,Anand K. Muthusamy,Yajie Liang,Timothy A. Brown,William C. Lemon,Ronak Patel,Rongwen Lu,John J. Macklin,Philipp J. Keller,Na Ji,Luke D. Lavis +10 more
TL;DR: This work refined and extended the Janelia Fluor strategy, finding that incorporation of 3-substituted azetidine groups allows rational tuning of the spectral and chemical properties of rhodamine dyes with unprecedented precision.
Journal ArticleDOI
Imaging live-cell dynamics and structure at the single-molecule level.
TL;DR: This review discusses the concepts and strategies of structural and functional imaging in living cells at the single-molecule level with minimal perturbations to the specimen.