Pilar Fonseca

TL;DR: The Crc protein is a translational repressor that recognizes a specific target at some mRNAs, controlling catabolite repression and co‐ordinating carbon metabolism in pseudomonads, and it is proposed that CrcZ and CrcY act in concert, sequestering and modulating the levels of free Crc according to metabolic conditions.

TL;DR: Understanding of the P. putida lifestyle at low temperature is improved, with changes seemed directed towards neutralizing problems created by low temperature, such as increased protein misfolding, the increased stability of DNA/RNA secondary structures, reduced membrane fluidity and a reduced growth rate.

TL;DR: It is proposed that Crc controls TOL genes in two ways: first, Crc inhibits the translation of the XylR and XylS regulators, thereby reducing the transcription of all TOL pathway genes, and second, CrC inhibits thetranslation of specific structural genes of the pathway, acting mainly on proteins involved in the first steps of toluene assimilation.

TL;DR: Interestingly, whereas the changes of gene transcription were widespread, the cell physiology was hardly affected, which illustrates how efficient reorganization of the gene transcription is for dealing with environmental changes that may otherwise be harmful.

TL;DR: The present work shows that when cells grow exponentially at 10°C, the repressive effect of Crc on many genes is significantly reduced compared with that seen at 30°C; this might help understanding the behaviour of this bacterium in bioremediations or rhizoremediation strategies at low temperatures.