Piyada Juntawong

TL;DR: The precise mapping of ribosome footprints on mRNAs is used to investigate translational regulation under control and sublethal hypoxia stress conditions in seedlings of Arabidopsis thaliana and illuminates prevalent and nuanced regulation of protein synthesis under optimal and energy-limiting conditions.

TL;DR: Parts of submergence/waterlogging stress that have not yet been thoroughly studied at the molecular level in this context are highlighted, such as miRNA and retrotransposon expression, the influence of light/dark cycles, protein isoforms, root architecture, sugar sensing and signaling, post-stress molecular events, heavy-metal and salinity stress, and mRNA dynamics.

TL;DR: Methods for the isolation and quantification of polysome complexes from plant tissues and a modification of the conventional polysome isolation procedure is described for transgenic Arabidopsis thaliana that express an epitope-tagged version of ribosomal protein L18 (RPL18) that facilitates capture of Ribosomes from crude cell extracts by a one-step immunoprecipitation method.

TL;DR: How quantitative assessment of mRNAs in distinct cytoplasmic mRNPs, such as polyribosomes (polysomes), has provided new perspectives on post-transcriptional regulation from the global to gene-specific level is reviewed.

TL;DR: It is proposed that modulation of translation of a subset of cellular mRNAs functions as an energy conservation mechanism in response to changes in oxygen availability.