R
R.J. Ashman
Researcher at University of Adelaide
Publications - 28
Citations - 1077
R.J. Ashman is an academic researcher from University of Adelaide. The author has contributed to research in topics: Blastocyst & Somatic cell nuclear transfer. The author has an hindex of 18, co-authored 28 publications receiving 1049 citations.
Papers
More filters
Journal ArticleDOI
Removal of cytoplasmic lipid enhances the tolerance of porcine embryos to chilling
Hiroshi Nagashima,Naomi Kashiwazaki,R.J. Ashman,Christopher G. Grupen,Robert F. Seamark,Mark B. Nottle +5 more
TL;DR: The data demonstrate that the sensitivity of porcine embryos to chilling is related to their high lipid content and that they can become tolerant to chilling if their lipid content is reduced.
Journal ArticleDOI
Production of Cloned Pigs from Cultured Fetal Fibroblast Cells
A.C. Boquest,Christopher G. Grupen,Sharon J. Harrison,Stephen M. McIlfatrick,R.J. Ashman,Anthony J. F. D'apice,Mark B. Nottle +6 more
TL;DR: Efficient reprogramming of porcine donor nuclei by fusing cells in the absence of calcium followed by chemical activation of recipient cytoplasts was reflected in high rates of development to blastocyst and pregnancy initiation leading to full term development.
Journal ArticleDOI
Use of adult mesenchymal stem cells isolated from bone marrow and blood for somatic cell nuclear transfer in pigs.
Renate Faast,Sharon J. Harrison,Luke F.S. Beebe,Stephen M. McIlfatrick,R.J. Ashman,Mark B. Nottle +5 more
TL;DR: This is the first study to know, to report that MSCs can be derived from peripheral blood and used for SCNT for any species, and may provide an alternative cell type for cloning live animals.
Journal ArticleDOI
Production of homozygous α‐1,3‐galactosyltransferase knockout pigs by breeding and somatic cell nuclear transfer
Mark B. Nottle,Luke F.S. Beebe,Sharon J. Harrison,Stephen M. McIlfatrick,R.J. Ashman,P. J. O'connell,Evelyn J Salvaris,Nella Fisicaro,Sandra Pommey,Peter J. Cowan,Anthony J F D'Apice +10 more
TL;DR: It is demonstrated that Gal KO pigs can be produced by breeding with each other and by SCNT using Gal KO fetal fibroblasts.
Journal ArticleDOI
Relationship between donor animal age, follicular fluid steroid content and oocyte developmental competence in the pig
Christopher G. Grupen,Stephen M. McIlfatrick,R.J. Ashman,A.C. Boquest,David T. Armstrong,Mark B. Nottle +5 more
TL;DR: The results support the hypothesis that a greater proportion of adult oocytes than of prepubertal oocytes has completed 'oocyte capacitation', and the differences in FF steroid content are indicative of the different follicular environments from which the prepUbertal and adult oocyte were isolated.