A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. Coli I. Purification and characterization of alkaline phosphatase

The majority (>95%) of organic matter in aquatic environments is composed of polymeric, high-molecular-weight compounds (Allen, 1976; Romankevich, 1984; Cole et al., 1984; Thurman. 1985; Munster and Chrost, 1990). Because the passage of organic molecules across the microbial cytoplasmic membrane is an active process requiring specific transport enzymes (permeases), only small (low-molecular-weight) and simple molecules can be directly transferred from the environment into the cell (Rogers, 1961; Payne, 1980a; Geller, 1985). This means that only a small portion of the total dissolved organic matter (DOM) is readily utilizable in natural waters (Munster, 1985; Azam and Cho, 1987; Jorgensen, 1987), and that the majority of DOM cannot be directly transported to microbial cells because of the large size of its molecules.

Environmental Control of the Synthesis and Activity of Aquatic Microbial Ectoenzymes

The use of an enzyme as a label has a number of advantages over the use of other labels in both immunohistochemistry and immunoassay. Immunofluorescence techniques are not suitable for ultrastructural research on cells, and ferritin-labeled antibodies allow only electronmicroscopic studies. By contrast, enzyme-labeled antibodies permit localization of cellular antigens in relation to tissue structures under light microscope and also demonstration of cellular antigens at an ultrastructural level by electronmicroscopy. Antibody or antibody fragments labeled with enzymes of small molecular weight can more readily permeate cells of tissue sections than ferritin-labeled antibodies. The color of tissue sections prepared by immunoenzymatic techniques is stable for years, while immunofluorescence of tissue sections decreases rapidly when exposed to light. Radioisotope-labeled reagents decay with time; there are health hazards due to radio isotopes; and disposal of radioactive waste is becoming increasing...

Enzyme-labeling of antibodies and their fragments for enzyme immunoassay and immunohistochemical staining.

Review of the progress of dairy science: bovine mastitis: milk compositional changes and related diagnostic tests.

The milk fat globule membrane.

The purification of alkaline phosphatases of animal tissues

The lipoprotein particles in cow's milk.

The substrate specificity and inhibition of alkaline phosphatases of cow's milk and calf intestinal mucosa.

The kinetics of hydrolysis of phenyl phosphate by alkaline phosphatases

Some properties of alkaline phosphatase of cow's milk and calf intestinal mucosa.

R. K. Morton

Papers

The purification of alkaline phosphatases of animal tissues

The lipoprotein particles in cow's milk.

The substrate specificity and inhibition of alkaline phosphatases of cow's milk and calf intestinal mucosa.

The kinetics of hydrolysis of phenyl phosphate by alkaline phosphatases

Some properties of alkaline phosphatase of cow's milk and calf intestinal mucosa.